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首页> 外文期刊>Cell biology international. >Bcl-2 and caspase-8 related anoikis resistance in human osteosarcoma MG-63 cells.
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Bcl-2 and caspase-8 related anoikis resistance in human osteosarcoma MG-63 cells.

机译：Bcl-2和caspase-8与人骨肉瘤MG-63细胞中的失神经相关。

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摘要

Detachment of adherent cells from extracellular matrix results in apoptosis, a process termed "anoikis". Resistance to anoikis is implicated in the progression of many malignancies by facilitating the migration and eventual colonization of distant sites. Human kidney epithelial cells 293T, human osteoblast cells hFOB 1.19 and human osteosarcoma cells Saos-2 significantly underwent anoikis when adherence was prevented. But human osteosarcoma MG-63 cells were distinctly anoikis resistant when detached. They formed large aggregates and showed little apoptosis compared to the other cells. When MG-63 cells were in suspension, caspase-8, physically associated with death receptor was activated by cell-matrix detachment, whereas. Caspase-3 and caspase-9 were not activated. Translational level of Bcl-2 significantly increased in a time-dependent manner, but the level of beta-catenin and PI3K did not. Caspase-8 participates in an anoikis-inducing process in MG-63 cells at an early time, and overexpression of Bcl-2 blocks activation of caspase-8 making MG-63 cells anoikis resistant.
机译：从细胞外基质中分离粘附细胞会导致细胞凋亡，这一过程称为“无神经”。通过促进远处部位的迁移和最终定殖，对无神经症的抗性与许多恶性肿瘤的发展有关。预防粘连时，人肾上皮细胞293T，人成骨细胞hFOB 1.19和人骨肉瘤细胞Saos-2显着地发生了无神经。但是，人骨肉瘤MG-63细胞在分离时具有明显的无阳极抗性。与其他细胞相比，它们形成了大的聚集体，几乎没有凋亡。当MG-63细胞悬浮时，与死亡受体物理相关的胱天蛋白酶8被细胞-基质脱离激活。 Caspase-3和caspase-9未激活。 Bcl-2的翻译水平以时间依赖性的方式显着增加，但β-catenin和PI3K的水平没有。 Caspase-8早期参与了MG-63细胞的阳极诱导过程，Bcl-2的过表达阻止了caspase-8的活化，使MG-63细胞具有阳极抗性。

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