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首页> 外文期刊>Cell biology international. >Aggregatibacter actinomycetemcomitans lipopolysaccharide affects human gingival fibroblast cytoskeletal organization
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Aggregatibacter actinomycetemcomitans lipopolysaccharide affects human gingival fibroblast cytoskeletal organization

机译：聚合杆菌放线杆菌脂多糖影响人牙龈成纤维细胞的细胞骨架

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摘要

The cytoskeleton is a dynamic structure that plays a key role in maintaining cell morphology and function. This study investigates the effect of bacterial wall lipopolysaccharide (LPS), a strong inflammatory agent, on the dynamics and organization of actin, tubulin, vimentin, and vinculin proteins in human gingival fibroblasts (HGF). A time-dependent study showed a noticeable change in actin architecture after 1.5 h of incubation with LPS (1 μg/ml) with the formation of orthogonal fibers and further accumulation of actin filament at the cell periphery by 24 h. When 0.01–10 μg/ml of LPS was added to human gingival fibroblast cultures, cells acquired a round, flat shape and gradually developed cytoplasmic ruffling. Lipopolysaccharides extracted from Aggregatibacter actinomycetemcomitans periodontopathogenic bacteria promoted alterations in F-actin stress fibres of human gingival cells. Normally, human gingival cells have F-actin fibres that are organized in linear distribution throughout the cells, extending along the cell's length. LPS-treated cells exhibited changes in cytoskeletal protein organization, and F-actin was reorganized by the formation of bundles underneath and parallel to the cell membrane. We also found the reorganization of the vimentin network into vimentin bundling after 1.5 h of treatment. HGF cells exhibited diffuse and granular γ-tubulin stain. There was no change in LPS-treated HGF. However, vinculin plaques distributed in the cell body diminished after LPS treatment.
机译：细胞骨架是动态结构，在维持细胞形态和功能中起关键作用。这项研究调查了细菌壁脂多糖（LPS）（一种强力的发炎剂）对人牙龈成纤维细胞（HGF）中肌动蛋白，微管蛋白，波形蛋白和纽蛋白的动力学和组织的影响。一项与时间有关的研究表明，与LPS（1μg/ ml）孵育1.5小时后，肌动蛋白结构发生了显着变化，并形成了正交纤维，并在24 h时肌动蛋白丝进一步积聚在细胞周围。当向人牙龈成纤维细胞培养物中添加0.01-10μg/ ml的LPS时，细胞会呈圆形，扁平状并逐渐发展成胞质波纹。从牙线放线杆菌的牙周病原菌中提取的脂多糖促进人牙龈细胞F-肌动蛋白应激纤维的改变。通常，人牙龈细胞具有F-肌动蛋白纤维，这些纤维在整个细胞中呈线性分布，沿着细胞的长度延伸。经LPS处理的细胞在细胞骨架蛋白组织中表现出变化，而F-肌动蛋白则通过在细胞膜下方和与细胞膜平行的束形成而重新组织。我们还发现在处理1.5小时后，波形蛋白网络重组为波形蛋白束。 HGF细胞表现出弥散性和颗粒状的γ-微管蛋白染色。经LPS处理的HGF没有变化。然而，LPS处理后分布在细胞体内的纽蛋白斑减少。

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