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首页> 外文期刊>Cell biology international. >Methods for rearing Mesostoma ehrenbergii in the laboratory for cell biology experiments, including identification of factors that influence production of different egg types.
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Methods for rearing Mesostoma ehrenbergii in the laboratory for cell biology experiments, including identification of factors that influence production of different egg types.

机译：在实验室中饲养埃伦氏肉芽肿以进行细胞生物学实验的方法，包括确定影响不同蛋类型生产的因素。

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Mesostoma ehrenbergii spermatocytes are uniquely useful to study various aspects of cell division. Their chromosomes are large in size and few in number, with only three bivalent and four univalent chromosomes. During prometaphase, bipolar bivalents oscillate regularly to and from the poles for 1-2 hours. The univalents remain at the poles but occasionally move from one pole to the other. In addition, a precocious cleavage furrow forms during prometaphase and remains partially constricted until anaphase. Attempts to rear these animals indefinitely in laboratory conditions, however, have been mostly unsuccessful because of their reproductive strategy. M. ehrenbergii are hermaphroditic flatworms that can produce viviparous offspring (termed S eggs) and/or diapausing eggs (termed D eggs) and they follow either one of two reproductive patterns: (1) they first form S eggs and following the delivery of these eggs produce D eggs, or (2) they only produce D eggs. When only D eggs are formed, which is common under laboratory conditions, the stocks die out until the D eggs hatch, which is irregular and creates unpredictable wait times. Consequently, in order to maintain M. ehrenbergii stocks to study their spermatocytes, we examined various factors that might influence egg-type production. Feeding them daily and keeping them at 25°C favours S egg production. Currently, our cultures have reached the 53rd generation. We herein describe our rearing and dissection methods, and some experiments which led to our present rearing methods.
机译：艾伦氏肉芽肿的精母细胞对研究细胞分裂的各个方面非常有用。它们的染色体很大，数目很少，只有三价和四价单价染色体。在前中期，双极二价离子在两极之间有规律地振荡1-2小时。单价原子保留在两极，但偶尔会从一个极移到另一极。另外，早熟的犁沟形成于前中期，并保持部分收缩直至后期。但是，由于它们的繁殖策略，在实验室条件下无限期饲养这些动物的尝试大多没有成功。 ehrenbergii属于雌雄同体的flat虫，可以产生胎生子代（称为S卵）和/或滞育卵（称为D卵），它们遵循两种繁殖方式中的一种：（1）它们首先形成S卵，然后将其繁殖卵产生D卵，或者（2）他们只产生D卵。当仅形成D蛋时（这在实验室条件下很常见），库存会消失，直到D蛋孵化为止（这是不规则的，并导致无法预测的等待时间）。因此，为了维持埃伦伯格氏菌的储备以研究其精母细胞，我们研究了可能影响卵型生产的各种因素。每天喂养它们并保持在25°C有利于S卵的生产。目前，我们的文化已发展到第53代。我们在这里描述了我们的饲养和解剖方法，以及一些导致我们目前的饲养方法的实验。

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