...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Hepatic differentiation of mouse ES cells into BE cells in vitro.
【24h】

Hepatic differentiation of mouse ES cells into BE cells in vitro.

机译：体外将小鼠ES细胞肝分化为BE细胞。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To date, the hepatic differentiation of embryonic stem (ES) cells into biliary epithelial (BE) cells has only been identified in hepatocytes. In this study, an attempt was made to induce the differentiation of ES cells to BE cells. In order to induce hepatic, and then BE cell differentiation, growth factors such as TGF, FGF, HGF and EGF were added to the culture medium supplied to embryonic bodies (EBs) that were derived from ES cells. The marker genes and corresponding proteins of hepatocytes and BE cells such as AFP, ALB, CK8, CK18, CK7, CK19 and GGT, etc. were detected by reverse transcriptase-polymerase chain reaction (RT-PCR), immunocytochemistry (ICC) and enzymatic histochemistry. Lastly, the ratio of BE-like cells to all EBs cells was analyzed and determined by flow cytometry (FCM). Hepatocyte and BE cell marker genes and proteins were found to be expressed in the cytoplasm of differentiated cells. On day 10 of differentiation, many round structures appeared in the EBs culture system and the marker proteins of BE cells were found to be expressed in these structures. The BE cell differentiation ratio continually increased from its initial value of 1.7% on day 13 to 7.4% on day 21. ES cells were found to be able to differentiate into BE cells when cultured using medium with appropriate cell growth factors. These BE cells may be a novel source of differentiated cell types for liver engineering.
机译：迄今为止，仅在肝细胞中鉴定出胚胎干（ES）细胞向胆汁上皮（BE）细胞的肝分化。在该研究中，尝试诱导ES细胞向BE细胞的分化。为了诱导肝细胞分化，然后诱导BE细胞分化，将生长因子（例如TGF，FGF，HGF和EGF）添加到培养基中，该培养基提供给源自ES细胞的胚胎体（EB）。通过逆转录-聚合酶链反应（RT-PCR），免疫细胞化学（ICC）和酶法检测肝细胞和BE细胞的标志物基因和相应的蛋白质，例如AFP，ALB，CK8，CK18，CK7，CK19和GGT等。组织化学。最后，通过流式细胞术（FCM）分析和确定BE样细胞与所有EBs细胞的比例。发现肝细胞和BE细胞标记基因和蛋白质在分化细胞的细胞质中表达。在分化的第10天，EBs培养系统中出现了许多圆形结构，并且发现BE细胞的标记蛋白在这些结构中表达。 BE细胞分化率从第13天的初始值的1.7％连续增加到第21天的7.4％。发现当使用具有适当细胞生长因子的培养基培养时，ES细胞能够分化为BE细胞。这些BE细胞可能是肝工程中分化细胞类型的新来源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号