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首页> 外文期刊>Cell biology international. >Effects of overexpression of Sim2 on spatial memory and expression of synapsin I in rat hippocampus.
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Effects of overexpression of Sim2 on spatial memory and expression of synapsin I in rat hippocampus.

机译：Sim2的过表达对大鼠海马空间记忆和突触蛋白I表达的影响。

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摘要

The single-minded 2 gene (Sim2) plays a crucial role in the mental retardation of Down Syndrome (DS). To explore how Sim2 influences spatial memory, a DNA plasmid-encoding mouse Sim2 (mSim2) wrapped with liposome was bilaterally injected into the hippocampus of rats. The effect of overexpressing mSim2 on spatial learning was determined by a Morris water maze task. The expression of synapsin I was detected by reverse transcriptional-polymerase chain reaction (RT-PCR) analysis and immunohistochemistry, respectively. The phosphosynapsin was also examined by immunohistochemistry. As demonstrated by RT-PCR, mSim2 was overexpressed in the hippocampus of rats, and pcDNA3/mSim2-transfected rats showed longer latency to find the hidden platform compared with pcDNA3-transfected rats (P<0.05). Synapsin I mRNA and protein expression were decreased significantly by mSim2 transfection, as demonstrated by RT-PCR and immunohistochemistry. Moreover, the expression profile of phosphosynapsin was similar to that of synapsin I. So it is concluded that Sim2 could impair the ability of learning and memory by inhibiting synaptic plasticity, and may play a crucial role in the pathogenesis of DS.
机译：一心一意的2基因（Sim2）在唐氏综合症（DS）的智力发育迟缓中起着至关重要的作用。为了探索Sim2如何影响空间记忆，将包裹脂质体的DNA编码小鼠Sim2（mSim2）DNA双向注入大鼠海马。莫里斯水迷宫任务确定了过表达mSim2对空间学习的影响。分别通过逆转录-聚合酶链反应（RT-PCR）分析和免疫组化检测突触素I的表达。还通过免疫组织化学检查了磷酸突触蛋白。通过RT-PCR证实，mSim2在大鼠海马中过表达，与pcDNA3转染的大鼠相比，pcDNA3 / mSim2转染的大鼠发现隐藏平台的潜伏期更长（P <0.05）。 RT-PCR和免疫组织化学证实，mSim2转染可显着降低突触蛋白I mRNA和蛋白表达。此外，磷酸突触素的表达谱与突触素I相似。因此可以得出结论，Sim2可能通过抑制突触可塑性来损害学习和记忆能力，并可能在DS的发病机制中起关键作用。

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