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Angiogenic potential difference between two types of endothelial progenitor cells from human umbilical cord blood.

机译:人类脐带血中两种内皮祖细胞之间的血管生成潜能差异。

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The hierarchy of endothelial progenitor cells (EPCs) in human umbilical cord blood has been disclosed. In this study we compare, for the first time, the angiogenic potential difference between two types of EPCs. We cultured mononuclear cells (MNCs) isolated from human umbilical cord blood using endothelial cell-conditioned medium and obtained two types of EPCs, referred to as circulating angiogenic cells (CACs) and high proliferative potential endothelial progenitor cells (HPP-EPCs). Both types of cells possess characteristics of EPCs, including expressing CD31, VE-cadherin, KDR and von Willebrand factor, uptake of Ac-LDL and binding to lectin. However, unlike CACs, which express CD14 but not CD133, HPP-EPCs express CD133 but not CD14. Also, unlike CACs, HPP-EPCs display stronger proliferation and clonogenic potential in vitro and show stronger ability to promote vascular growth in the hind-limb model of ischemia in mice (BALB/C-nu) in vivo.
机译:已经公开了人脐带血中内皮祖细胞(EPC)的层次。在这项研究中,我们首次比较了两种类型的EPC之间的血管生成电位差。我们使用内皮细胞条件培养基培养了从人脐带血中分离的单核细胞(MNC),并获得了两种类型的EPC,称为循环血管生成细胞(CAC)和具有高增殖潜能的内皮祖细胞(HPP-EPC)。两种类型的细胞都具有EPCs的特征,包括表达CD31,VE-钙黏着蛋白,KDR和von Willebrand因子,Ac-LDL的摄取以及与凝集素的结合。但是,与表达CD14但不表达CD133的CAC不同,HPP-EPC表达CD133而不表达CD14。而且,与CAC不同,HPP-EPC在体外显示出更强的增殖和克隆形成潜力,并且在体内小鼠的后肢缺血模型(BALB / C-nu)中显示出较强的促进血管生长的能力。

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