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首页> 外文期刊>Cell biology international. >Pre-adipocytes commitment to neurogenesis 1: preliminary localisation of cholinergic molecules.
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Pre-adipocytes commitment to neurogenesis 1: preliminary localisation of cholinergic molecules.

机译：前脂肪细胞对神经发生的承诺1：胆碱能分子的初步定位。

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摘要

A great effort has recently been made to obtain human stem cells able to differentiate into cholinergic neurons, as a number of diseases are associated to the cholinergic neuron loss, degeneration or incorrect function (Alzheimer's disease and motor neuron disease). A stem cell population (i.e. pre-adipocytes) is present in the adipose stromal compartment. Pre-adipocytes, like the mesodermic derivative cells, retain high plasticity and potentiality to convert in vitro from one phenotype into many others, and they can be isolated from adult adipose tissue. Pre-adipocytes committed in vitro to neural differentiation were followed up to the acquisition of neural morphology. Acetylcholinesterase and choline acetyltransferase are expressed from the native cell stage, with different localisations and roles during neural commitment. Western blots show the beginning of a new synthesis of these enzymes at 4 weeks of culture of neurogenic pre-adipocytes, in parallel with neural morphology. The passage of the choline-acetyltransferase immunoreactivity from cytoplasmic to membrane localisation shows the possible onset of catalytic activity and the histochemical reaction confirms the activity of acetylcholinesterase. This explains the possibility of obtaining cholinergic-like phenotype from pre-adipocytes.
机译：最近，人们为获得能够分化为胆碱能神经元的人类干细胞付出了巨大的努力，因为许多疾病都与胆碱能神经元的丧失，变性或功能失调有关（阿尔茨海默氏病和运动神经元疾病）。脂肪基质区室中存在干细胞群体（即前脂肪细胞）。像中皮衍生细胞一样，前脂肪细胞具有很高的可塑性和潜力，可以在体外从一种表型转化为许多其他表型，并且可以从成年脂肪组织中分离出来。对体外致力于神经分化的前脂肪细胞进行随访，直至获得神经形态。乙酰胆碱酯酶和胆碱乙酰基转移酶从天然细胞阶段表达，在神经活动过程中具有不同的定位和作用。 Western印迹显示，在培养神经源性前脂肪细胞4周后，与神经形态学平行，这些酶开始了新的合成。胆碱-乙酰转移酶免疫反应性从细胞质到膜定位的传递表明可能开始催化活性，并且组织化学反应证实了乙酰胆碱酯酶的活性。这解释了从前脂肪细胞获得胆碱能样表型的可能性。

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