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Protein kinase c in erythroleukaemia cells: temporal variations in the expression of the alpha, epsilon and zeta isoforms.

机译:红白血病细胞中的蛋白激酶c:α,ε和zeta亚型的表达随时间变化。

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摘要

Determinations of mRNA and enzyme protein analyses for the protein kinase C (PKC) isoforms, alpha, epsilon and zeta, representing the classical, novel and atypical groups, respectively, in murine erythroleukaemic (MEL) cells revealed the occurrence of cyclic behaviour in expression. Such rhythmicity was not apparent when multiple analyses using only a single time-point were considered; the importance of incorporating a time component into experimental design was demonstrated. On induction of differentiation over a period of five days with hexamethylene bisacetamide, changes in the patterns of expression of mRNA and protein were apparent, as compared with those of untreated, proliferating cells. Modulation of the dynamics of the various PKC isoforms may represent a regulatory mechanism for the control of a diversity of cellular functions. Copyright 2000 Academic Press.
机译:对鼠类红白血病(MEL)细胞中分别代表经典,新颖和非典型组的蛋白激酶C(PKC)同工型,α,ε和zeta的mRNA和酶蛋白分析的测定揭示了表达中循环行为的发生。当只考虑一个时间点进行多次分析时,这种节奏感并不明显。证明了将时间成分纳入实验设计的重要性。与未处理的增殖细胞相比,六亚甲基双乙酰胺在五天的时间内诱导分化,mRNA和蛋白质表达模式的变化是明显的。各种PKC同工型的动力学的调节可以代表用于控制细胞功能多样性的调节机制。版权所有2000学术出版社。

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