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首页> 外文期刊>Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis >Comparative multi-color flow cytometric analysis of cell surface antigens in bone marrow hematopoietic progenitors between refractory anemia and aplastic anemia.
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Comparative multi-color flow cytometric analysis of cell surface antigens in bone marrow hematopoietic progenitors between refractory anemia and aplastic anemia.

机译：难治性贫血和再生障碍性贫血之间的骨髓造血祖细胞表面抗原的比较多色流式细胞术分析。

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摘要

Refractory anemia (RA) in myelodysplastic syndrome (MDS) without prominent dysplasia closely resemble the mild type of aplastic anemia (AA) in their hematological features. This sometimes makes it difficult to distinguish clearly between the two diseases. Using the multi-color flow cytometric technique, we compared cell surface antigen expression patterns on bone marrow hematopoietic progenitor cells which were isolated as a CD34 positive- CD45 dull positive with low side scatter intensity (CD34(+)CD45(dull+)SSC(low)) population in flow cytogram between RA (n=12) and AA (n=11). The antigens analyzed in CD34(+)CD45(dull+)SSC(low) mononuclear cells were: CD38 and CD71 for cell growth-related antigens, CD 33 and CD13 for myeloid and monocytoid lineage-associated antigens, CD7 and CD19 for lymphoid lineage, and CD14 for a monocytic lineage specific antigen. The percentages of CD34(+)CD45(dull+)SSC(low) cells in bone marrow non-erythroid mononuclear cells, and the expression frequencies of CD38, CD71, CD33 and CD13 antigens in CD34(+)CD45(dull+)SSC(low) progenitors were all significantly decreased in AA compared to normal bone marrows (n=7) (P<0.005). In contrast, in RA bone marrows the percentages of CD34(+)CD45(dull+)SSC(low) cells showed wide distribution and the cell surface antigen expression patterns varied among each case: some cases showed low frequencies of CD38 and CD71 expression as well as AA, whereas the others showed high expression frequency of specific antigen(s) which may reflect the clonal expansion of an abnormal clone in bone marrow. An MDS patient who had progressed from RA to RAEB showed further projecting pattern of expression of CD38 and CD33 in CD34(+)CD45(dull+)SSC(low) population in accordance with the disease progression. These data suggest that analysis of cell surface antigen expression patterns of CD34(+)CD45(dull+)SSC(low) progenitor cells by multi-color flow cytometry appears to be a useful method for qualitative and quantitative assessment of marrow progenitor states in AA and RA, therefore this method could be helpful for early detection of clonal evolution in MDS.
机译：没有明显异常增生的骨髓增生异常综合征（MDS）中的难治性贫血（RA）在血液学特征上与轻度再生障碍性贫血（AA）非常相似。有时这使得很难清楚地区分这两种疾病。使用多色流式细胞仪技术，我们比较了骨髓造血祖细胞上的细胞表面抗原表达模式，这些细胞被分离为CD34阳性-CD45钝阳性且具有低侧向散射强度（CD34（+）CD45（dull +）SSC（低））RA（n = 12）和AA（n = 11）之间的流式细胞术图人口。在CD34（+）CD45（dull +）SSC（低）单核细胞中分析的抗原为：CD38和CD71用于细胞生长相关抗原，CD 33和CD13用于髓样和单细胞系谱系相关抗原，CD7和CD19用于淋巴谱系， CD14为单核细胞系特异性抗原。骨髓非红系单核细胞中CD34（+）CD45（dull +）SSC（low）细胞的百分比以及CD34（+）CD45（dull +）SSC（low）中CD38，CD71，CD33和CD13抗原的表达频率）与正常骨髓相比，祖细胞的AA均显着降低（n = 7）（P <0.005）。相反，在RA骨髓中，CD34（+）CD45（dull +）SSC（low）细胞的百分比显示出较宽的分布，并且每种情况下细胞表面抗原的表达模式各不相同：某些情况下，CD38和CD71的表达频率也较低如AA所示，其他抗原则显示特定抗原的高表达频率，这可能反映了异常克隆在骨髓中的克隆扩增。从RA进展为RAEB的MDS患者根据疾病进展显示出CD34（+）CD45（dull +）SSC（低）人群中CD38和CD33的进一步表达模式。这些数据表明，通过多色流式细胞术分析CD34（+）CD45（dull +）SSC（low）祖细胞的细胞表面抗原表达模式似乎是一种定性和定量评估AA和MSC骨髓祖细胞状态的有用方法。 RA，因此该方法可能有助于早期检测MDS中的克隆进化。

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