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ENDOSCOPY: Coherent Raman scanning-fiber endoscope images at 7 fps

机译:内窥镜:7 fps的相干拉曼扫描纤维内窥镜图像

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Both stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy use light from a pump and probe laser beam scanned over tissue or a test sample to accomplish chemically selective, label-free, high-spatial-resolution imaging at up to video rates. These coherent Raman laser-scanning microscopes were not able to be miniaturized easily for endoscopy. But researchers at Harvard University (Cambridge, MA) and the University of Washington (Seattle, WA) have succeeded in developing a coherent Raman scanning-fiber endoscope, enabling SRS and CARS microscopy for in situ biological tissue analysis. In the standard coherent Raman scattering test setup, a 1064 nm neodymium:yttrium vanadate (Nd:YVO_(4)) laser provides 80 MHz repetition rate, 7 ps Stokes (probe-beam) pulses. A portion of this output is frequency doubled and sent to an optical parametric oscillator (OPO) to produce the pump beam at 800 nm. The Stokes beam is modulated and combined with the pump beam via a dichroic mirror. The beams are then linearly polarized and properly oriented for delivery along the proper axis of a 1-m-long section of polarization-maintaining (PM) optical fiber.
机译:受激拉曼散射(SRS)和相干抗斯托克斯拉曼散射(CARS)显微镜均使用泵浦发出的光,并探测在组织或测试样品上扫描的激光束,以实现化学选择性,无标记,高空间分辨率的成像最高视频速率。这些相干的拉曼激光扫描显微镜无法轻易地小型化用于内窥镜检查。但是,哈佛大学(马萨诸塞州剑桥市)和华盛顿大学(华盛顿州西雅图市)的研究人员已经成功地开发出了一种相干的拉曼扫描纤维内窥镜,使SRS和CARS显微镜能够进行原位生物组织分析。在标准相干拉曼散射测试设置中,使用1064 nm钕钕钒酸盐(Nd:YVO_(4))激光器可提供80 MHz的重复频率,7 ps斯托克斯(探测光束)脉冲。该输出的一部分倍频,然后发送到光学参量振荡器(OPO),以产生800 nm的泵浦光束。斯托克斯光束经过分色镜调制并与泵浦光束合并。然后,将光束线性偏振并正确定向,以便沿着偏振保持(PM)光纤的1米长部分的正确轴传输。

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