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首页> 外文期刊>Cell motility and the cytoskeleton >Cytoskeletal asymmetry in Zea mays subsidiary cell mother cells: a monopolar prophase microtubule half-spindle anchors the nucleus to its polar position.
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Cytoskeletal asymmetry in Zea mays subsidiary cell mother cells: a monopolar prophase microtubule half-spindle anchors the nucleus to its polar position.

机译：玉米（Zea mays）辅助细胞母细胞中的细胞骨架不对称：单极前期微管半纺锤将细胞核锚定在其极性位置。

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摘要

Double labeling of microtubules and actin filaments revealed that in prophase subsidiary mother cells of Zea mays a monopolar prophase microtubule "half-spindle" is formed, which lines the nuclear hemisphere distal to the inducing guard mother cell. The nuclear hemisphere proximal to the guard mother cell is lined by an F-actin cap, consisting of a cortical F-actin patch and actin filaments originating from it. The microtubules of the "half-spindle" decline from the nuclear surface and terminate to the preprophase microtubule band. After disintegration of the latter, a bipolar metaphase spindle is organized. The polar F-actin cap persists during mitosis and early cytokinesis, extending to the chromosomes and the subsidiary cell daughter nucleus. In oryzalin treated subsidiary mother cells the prophase nuclei move away from the polar site. Cytochalasin B and latrunculin-B block the polar migration of subsidiary mother cell nuclei, but do not affect those already settled to the polar position. The prophase nuclei of latrunculin-B treated subsidiary mother cells are globally surrounded by microtubules, while the division plane of latrunculin-B treated subsidiary mother cells is misaligned. The prophase nuclei of brick 1 mutant Zea mays subsidiary mother cells without F-actin patch are also globally surrounded by microtubules. The presented data show that the prophase microtubule "half-spindle"-preprophase band complex anchors the subsidiary mother cell nucleus to the polar cell site, while the polar F-actin cap stabilizes the one metaphase spindle pole proximal to the inducing guard mother cell.
机译：对微管和肌动蛋白丝的双重标记显示，在Zea的前期辅助母细胞中，可能会形成单极前期微管“半梭形”，其在诱导半球保护母细胞的远端排列着核半球。保护母细胞近端的核半球衬有F-肌动蛋白帽，该帽由皮质F-肌动蛋白贴片和源自其的肌动蛋白丝组成。 “半轴”的微管从核表面下降并终止于前期微管带。后者解体后，组织了双极中期纺锤体。极性F-肌动蛋白帽在有丝分裂和早期胞质分裂期间持续存在，延伸到染色体和辅助细胞子核。在用稻草素处理的辅助母细胞中，前期核移离极性位点。细胞松弛素B和latrunculin-B阻止了辅助母细胞核的极性迁移，但不影响已经沉降到极性位置的子核。 latrunculin-B处理的辅助母细胞的前核被微管整体包围，而latrunculin-B处理的辅助母细胞的分裂平面未对齐。没有F-肌动蛋白补丁的砖1突变玉米玉米子母细胞的前期细胞核也被微管整体包围。所提供的数据表明，前期微管“半轴”-前期带复合物将辅助母细胞核锚定在极性细胞部位，而极性F-肌动蛋白帽稳定了一个接近诱导型保母细胞的中期纺锤体极。

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