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首页> 外文期刊>Cell motility and the cytoskeleton >The effect of combined hypergravity and micro-grooved surface topography on the behaviour of fibroblasts.
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The effect of combined hypergravity and micro-grooved surface topography on the behaviour of fibroblasts.

机译：超重力和微槽表面形貌相结合对成纤维细胞行为的影响。

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摘要

This study evaluated in vitro the differences in morphological behaviour between fibroblast cultured on smooth and micro-grooved substrata (groove depth: 1 mum, width: 1, 2, 5, 10 microm), which undergo artificial hypergravity by centrifugation (10, 24 and 50 g; or 1 g control). The aim of the study was to clarify which of these parameters was more important to determine cell behaviour. Morphological characteristics were investigated using scanning electron microscopy and fluorescence microscopy in order to obtain qualitative information on cell spreading and alignment. Confocal laser scanning microscopy visualised distribution of actin filaments and vinculin anchoring points through immunostaining. Finally, expression of collagen type I, fibronectin, and alpha(1)- and beta(1)-integrin were investigated by PCR. Microscopy and image analysis showed that the fibroblasts aligned along the groove direction on all textured surfaces. On the smooth substrata (control), cells spread out in a random fashion. The alignment of cells cultured on grooved surfaces increased with higher g-forces until a peak value at 25 g. An ANOVA was performed on the data, for all main parameters: topography, gravity force, and time. In this analysis, all parameters proved significant. In addition, most gene levels were reduced by hypergravity. Still, collagen type 1 and fibronectin are seemingly unaffected by time or force. From our data it is concluded that the fibroblasts primarily adjust their shape according to morphological environmental cues like substratum surface whilst a secondary, but significant, role is played by hypergravity forces.
机译：这项研究在体外评估了平滑和微槽基质（槽深度：1毫米，宽度：1、2、5、10微米）上培养的成纤维细胞之间的形态学差异，这些基质通过离心（10、24和10）进行人工超重力50克;或1克对照）。该研究的目的是阐明这些参数中的哪一个对确定细胞行为更重要。为了获得细胞扩散和排列的定性信息，使用扫描电子显微镜和荧光显微镜研究了形态特征。共聚焦激光扫描显微镜通过免疫染色可视化肌动蛋白丝和新蛋白锚定点的分布。最后，通过PCR研究了I型胶原，纤连蛋白以及α（1）-和β（1）-整联蛋白的表达。显微镜和图像分析表明，成纤维细胞在所有纹理表面上沿着凹槽方向排列。在平滑的基质（对照）上，细胞以随机方式扩散。带槽表面上培养的细胞的排列随着更高的g力而增加，直到达到25 g的峰值。对于所有主要参数：地形，重力和时间，对数据执行了ANOVA。在此分析中，所有参数均证明是有效的。另外，大多数基因水平由于超重力而降低。尽管如此，1型胶原蛋白和纤连蛋白似乎不受时间或力量的影响。从我们的数据得出的结论是，成纤维细胞主要根据形态环境线索（例如，基质表面）调整形状，而超重力作用则是次要的但重要的作用。

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