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首页> 外文期刊>Cell motility and the cytoskeleton >Shaggy/GSK-3beta kinase localizes to the centrosome and to specialized cytoskeletal structures in Drosophila.
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Shaggy/GSK-3beta kinase localizes to the centrosome and to specialized cytoskeletal structures in Drosophila.

机译：毛茸茸的/ GSK-3beta激酶位于果蝇中的中心体和专门的细胞骨架结构。

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摘要

The assembly of a functional bipolar mitotic spindle requires an exquisite regulation of microtubule behavior in time and space. To characterize new elements of this machinery we carried out a GFP based "protein trap" screen and selected fusion proteins which localized to the spindle apparatus. By this method we identified Shaggy, the Drosophila homologue of glycogen synthase kinase-3beta (GSK-3beta), as a component of centrosomes. GSK-3beta acting in the Wingless signaling pathway is involved in a vast range of developmental processes, from pattern formation to cell-fate specification, and is a key factor for cell proliferation in most animals. We exploited our Shaggy::GFP Drosophila line to analyze the subcellular localizations of GSK-3beta/Shaggy and shed light on its multiple roles during embryogenesis. We found that Shaggy becomes enriched transiently in a variety of specialized cytoskeletal structures of the embryo, including centrosomes throughout mitosis, suggesting that this kinase is involved in the regulation of many aspects of the cytoskeleton function.
机译：功能性双极有丝分裂纺锤体的组装需要对微管行为在时间和空间上的精确调节。为了表征该机器的新元件，我们进行了基于GFP的“蛋白陷阱”筛选，并选择了定位于纺锤体设备的融合蛋白。通过这种方法，我们确定了糖原合酶激酶3beta（GSK-3beta）的果蝇同源物Shaggy，是其中心体的组成部分。 GSK-3beta在无翅信号通路中发挥作用，涉及从模式形成到细胞命运规范的广泛发育过程，并且是大多数动物细胞增殖的关键因素。我们利用Shaggy :: GFP果蝇系分析了GSK-3beta / Shaggy的亚细胞定位，并阐明了其在胚胎发生过程中的多种作用。我们发现，Shaggy短暂地富集了胚胎的各种专门细胞骨架结构，包括整个有丝分裂期间的中心体，这表明该激酶参与了细胞骨架功能的许多方面的调节。

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