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首页> 外文期刊>Cell biology international. >PRODUCTION OF GERMLINE CHIMERIC CHICKENS BY TRANSFER OF CULTURED PRIMORDIAL GERM CELLS
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PRODUCTION OF GERMLINE CHIMERIC CHICKENS BY TRANSFER OF CULTURED PRIMORDIAL GERM CELLS

机译：通过转移培养的原代生殖细胞生产胚芽嵌合小鸡

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Primordial germ cells (PGCs) from stage 27 (5.5-day-old) Korean native ogol chicken embryonic germinal ridges were cultured in vitro for 5 days. As in in vivo culture, these cultured PGCs were expected to have already passed beyond the migration stage. Approximately 200 of these PGCs were transferred into 2.5-day-old white leghorn embryonic blood stream, and then the recipient embryos were incubated until hatching. The rate of hatching was 58.8% in the manipulated eggs. Six out of 60 recipients were identified as germline chimeric chickens by their feather colour. The frequency of germline transmission of donor PGCs was 1.3-3.1% regardless of sex. The stage 27 PGCs will be very useful for collecting large numbers of PGCs, handling of exogenous DNA transfection during culture, and for the production of desired transgenic chickens. (C) 1997 Academic Press Limited. [References: 20]
机译：来自第27阶段（5.5天大）韩国本地ogol鸡胚胎生发脊的原始生殖细胞（PGC）在体外培养5天。如同在体内培养中一样，这些培养的​​PGC预计已超过迁移阶段。将这些PGC中的大约200个转移到2.5天大的白色来亨鸡胚胎血流中，然后将受体胚胎孵育直至孵化。操纵卵中的孵化率为58.8％。 60只接受者中有6只通过羽毛颜色被鉴定为种系嵌合鸡。不论性别，供体PGC的种系传播频率为1.3-3.1％。 27级PGC对收集大量PGC，在培养过程中处理外源DNA转染以及生产所需的转基因鸡非常有用。 （C）1997 Academic Press Limited。 [参考：20]

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