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首页> 外文期刊>Cell biology international. >Expression of glutamyl aminopeptidase by osteogenic induction in rat bone marrow stromal cells.
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Expression of glutamyl aminopeptidase by osteogenic induction in rat bone marrow stromal cells.

机译：通过成骨诱导在大鼠骨髓基质细胞中表达谷氨酰胺基肽酶。

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摘要

Glutamyl aminopeptidase (GluAP, EC 3.4.11.7, ENPEP) is a 130-kDa homodimeric zinc metallopeptidase which specifically cleaves the N-terminal glutamate or aspartate residue of peptidic substrates such as cholecystokinin-8 or angiotensin (Ang) II, in vitro. We used a DNA microarray hybridization (Genechip Rat Expression Array 230A, Affymetrix Inc., Santa Clara, CA, USA) to demonstrate that GluAP was upregulated in osteogenic induced rat bone marrow stromal cells (BMSCs). To compare the expression of GluAP in the osteogenic differentiation and non-osteogenic differentiation of rat BMSCs in vitro, the cells were osteogenic induced in vitro. We also performed an MTT assay, alkaline phosphatase assay, alizarin red staining, and an immunohistochemical analysis to determine the osteogenic differentiation of BMSCs. The expression of GluAP was examined by real-time polymerase chain reaction (PCR). The real-time PCR results showed that GluAP was upregulated in osteogenic differentiated BMSCs in vitro, suggesting that GluAP may be correlated with the osteogenic differentiation of BMSCs.
机译：谷氨酰胺基肽酶（GluAP，EC 3.4.11.7，ENPEP）是一种130 kDa的同型二聚体金属锌肽酶，可在体外特异性裂解肽底物如胆囊收缩素8或血管紧张素（Ang）II的N末端谷氨酸或天冬氨酸残基。我们使用DNA微阵列杂交（Genechip大鼠表达阵列230A，Affymetrix公司，美国加利福尼亚州圣克拉拉）来证明GluAP在成骨诱导的大鼠骨髓基质细胞（BMSCs）中上调。为了比较GluAP在大鼠BMSCs的成骨分化和非成骨分化中的表达，在体外对细胞进行了成骨诱导。我们还进行了MTT分析，碱性磷酸酶分析，茜素红染色和免疫组织化学分析，以确定BMSC的成骨分化。通过实时聚合酶链反应（PCR）检查GluAP的表达。实时荧光定量PCR结果表明，体外成骨分化的BMSCs中GluAP表达上调，提示GluAP可能与BMSCs的成骨分化有关。

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