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首页> 外文期刊>Cell biology international. >Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver.
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Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver.

机译:低氧治疗对能够迁移至受伤肝脏的骨髓细胞的影响。

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摘要

Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1alpha was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl(4)-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1alpha, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl(4) damaged liver.
机译:数量有限和再生性能差限制了成体干细胞的使用。我们测试了缺氧治疗作为增加细胞迁移的方法的效果。骨髓细胞(BMC)在0.1、3和20%的氧饱和度下培养24小时。缺氧处理后,凋亡分数的BMC降低。与在常氧条件下培养的细胞相比,在低氧处理的BMC中CXCR4的表达明显增加,并且它们对SDF-1alpha的迁移也得到了增强。缺氧的BMC比正常细胞具有更高程度的CCl(4)损伤的肝移植。 BMC的低氧治疗在减少那些细胞的凋亡以及增强向SDF-1alpha的细胞迁移方面具有优势,SDF-1alpha是与BMC结合的趋化因子表达CXCR4和受伤的组织,例如CCl(4)受损的肝脏。

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