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首页> 外文期刊>Cell biology international. >MICROJET IMPINGEMENT FOLLOWED BY SCANNING ELECTRON MICROSCOPY AS A QUALITATIVE TECHNIQUE TO COMPARE CELLULAR ADHESION TO VARIOUS BIOMATERIALS
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MICROJET IMPINGEMENT FOLLOWED BY SCANNING ELECTRON MICROSCOPY AS A QUALITATIVE TECHNIQUE TO COMPARE CELLULAR ADHESION TO VARIOUS BIOMATERIALS

机译：扫描电子显微镜后进行的微射流冲击作为定性技术比较细胞粘附到各种生物材料上

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摘要

Adhesion of cells to biomaterial surfaces is one of the major factors which mediates their biocompatibility. Quantitative or qualitative cell adhesion measurements would be useful for screening new implant materials. Microjet impingement has been evaluated by scanning electron microscopy, to determine to what extent it measures cell adhesion. The shear forces of the impingement, on the materials tested here, are seen to be greater than the cohesive strength of the cells in the impinged area, causing their rupture. The cell bodies are removed during impingement, leaving the sites of adhesion and other cellular material behind. Thus the method is shown not to provide quantification of cell adhesion forces for the metals and culture plastic tested. It is suggested that with highly adherent biomaterials, the distribution and patterns of these adhesion sites could be used for qualitative comparisons for screening of implant surfaces.
机译：细胞对生物材料表面的粘附是介导其生物相容性的主要因素之一。定量或定性的细胞粘附性测量将有助于筛选新的植入物材料。已通过扫描电子显微镜对微射流撞击进行了评估，以确定它在多大程度上测量了细胞粘附。可以看到，在此处测试的材料上，撞击的剪切力大于撞击区域中小室的内聚强度，从而导致其破裂。在撞击过程中，细胞体被去除，留下了粘附和其他细胞物质的位置。因此，显示该方法不能定量测试的金属和培养塑料的细胞粘附力。建议使用高度粘附的生物材料，这些粘附部位的分布和图案可用于对植入物表面进行筛选的定性比较。

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