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首页> 外文期刊>Cell biology international. >Effect of the pancreatic digestion with liberase versus collagenase on the yield, function and viability of neonatal rat pancreatic islets.
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Effect of the pancreatic digestion with liberase versus collagenase on the yield, function and viability of neonatal rat pancreatic islets.

机译：游离酶与胶原酶胰消化对新生大鼠胰岛的产量，功能和生存力的影响。

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摘要

Many obstacles hinder the clinical application of pancreatic islet transplantation as a cure for diabetes mellitus. One of them is the suitable isolation method of sufficient number of healthy islets for transplantation. In this context, liberase enzyme was developed as a purified form of the traditional collagenase. It was the aim of this study to investigate the effect of liberase-digestion on the yield, function and viability of neonatal rat islets, and to compare the new enzyme with the collagenase. Glucose-stimulated insulin secretion was measured as indication of the function, insulin content as indication for the synthetic activity of islet cells and DNA as an indication of cell viability. The results showed no difference between islets isolated either with collagenase or liberase. Glucose stimulated similarly the insulin secretion in both. Stimulation index tended, without significance, to be higher (55%) in liberase-isolated islets compared with the collagenase islets (49%). The viability of both was similar. The insulin synthesis (content) tended also to be better in liberase-isolated islets. It could be concluded that liberase could be non-significantly preferred in the isolation of neonatal rat islets in comparison with collagenase.
机译：许多障碍阻碍了胰岛移植作为糖尿病治疗的临床应用。其中之一是合适的分离方法，该方法具有足够数量的健康胰岛用于移植。在这种情况下，释放酶被开发为传统胶原酶的纯化形式。这项研究的目的是研究游离酶消化对新生大鼠胰岛产量，功能和生存力的影响，并将新酶与胶原酶进行比较。葡萄糖刺激的胰岛素分泌被测量为功能的指示，胰岛素含量被指示为胰岛细胞的合成活性的指示，并且DNA被指示为细胞生存力的指示。结果显示，用胶原酶或游离酶分离的胰岛之间没有差异。葡萄糖在两者中均类似地刺激胰岛素分泌。与胶原酶胰岛（49％）相比，游离酶分离的胰岛的刺激指数趋向于更高（55％）。两者的生存能力相似。在游离酶分离的胰岛中，胰岛素合成（含量）也趋向于更好。可以得出结论，与胶原酶相比，游离酶在新生大鼠胰岛的分离中可能不是非常重要的选择。

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