...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Secretion of oestrogen from murine-induced pluripotent stem cells co-cultured with ovarian granulosa cells in vitro.
【24h】

Secretion of oestrogen from murine-induced pluripotent stem cells co-cultured with ovarian granulosa cells in vitro.

机译：体外与卵巢颗粒细胞共培养的鼠诱导性多能干细胞分泌雌激素。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

POF (premature ovarian failure) is a distressing condition that is a common cause of infertility. No effective treatment is available to overcome the loss of fertility. A method to derive oestrogen from miPSCs (mouse-induced pluripotent stem cells) was explored as a potential treatment for POF. In this study, C57BL/6 female mice were injected with PMSG (pregnant mare's serum gonadotropin) to obtain ovarian GCs (granulosa cells) and then co-cultured with miPSCs. The morphological changes in the miPSCs co-cultured with GCs were observed by light microscopy. The expression of FSHR (follicle-stimulating hormone receptor) was detected by immunocytochemistry and flow cytometry. Radioimmunoassay was used to analyse the level of E2 (oestradiol) in culture supernatants. The results showed that the proportion of GCs expressing FSHR in GCs was over 90%. The E2 concentration of the culture supernatant of the GC group was 62.4 pg/ml on day 1 and decreased in a time-dependent manner. The opposite situation was observed in the miPSCs-GC co-cultured group with an E2 concentration of 87.9 pg/ml on day 1 that increased in a time-dependent manner to reach a concentration of 328.4 pg/ml on day 7. The data indicate that GC-like cells were effectively induced from miPSCs through indirect cell-to-cell contact. Our method provides a novel in vitro system to study miPSC differentiation, particularly the interactions between miPSCs and GCs. The ultimate goal of this approach would be to provide a treatment for POF in the future.
机译：POF（卵巢早衰）是一种令人不安的状况，是不孕的常见原因。没有有效的治疗方法可以克服生育能力的丧失。探索了一种从miPSC（小鼠诱导的多能干细胞）中提取雌激素的方法，作为POF的潜在治疗方法。在这项研究中，向C57BL / 6雌性小鼠注射PMSG（孕母马的血清促性腺激素）以获得卵巢GC（颗粒细胞），然后与miPSC共培养。通过光学显微镜观察与GC共培养的miPSC的形态变化。通过免疫细胞化学和流式细胞术检测FSHR（促卵泡激素受体）的表达。放射免疫分析法用于分析培养上清液中E2（雌二醇）的水平。结果表明，表达FSHR的GC在GC中的比例超过90％。 GC组培养上清液的E2浓度在第1天为62.4 pg / ml，并以时间依赖性方式降低。在miPSCs-GC共培养组中观察到相反的情况，在第1天的E2浓度为87.9 pg / ml，并随时间增加而在第7天达到328.4 pg / ml的浓度。数据表明通过间接细胞间接触从miPSC有效诱导了GC样细胞。我们的方法提供了一种新型的体外系统，用于研究miPSC的分化，特别是miPSC与GC之间的相互作用。这种方法的最终目标是将来为POF提供治疗。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号