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首页> 外文期刊>Cell biology international. >Dynein LIC1 localizes to the mitotic spindle and midbody and LIC2 localizes to spindle poles during cell division
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Dynein LIC1 localizes to the mitotic spindle and midbody and LIC2 localizes to spindle poles during cell division

机译:Dynein LIC1定位于有丝分裂纺锤体和中体,而LIC2定位于纺锤体极。

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摘要

CD-1 (cytoplasmic dynein-1) is a multisubunit motor protein complex involved in intracellular trafficking and mitosis. The dynein LIC (light intermediate chain)subunits, LIC1 (DLIC-1, gene symbol DYNC1LI1) and LIC2 (DLIC-2, gene symbol DYNC1LI2), associate with the dynein HC (heavy chain) in a mutually exclusive manner and thus define distinct functional CD-1 complexes. Here, we analysed the mitotic distribution of LIC1 and LIC2. We found that from metaphase through anaphase, LIC1 localizes to the mitotic spindle and concentrates within the midbody during the abscission step of cytokinesis. Conversely, LIC2 strongly localizes to the spindle poles from prophase through telophase. These data suggest distinct functions for LIC1 and LIC2-containing CD-1 complexes during cell division.
机译:CD-1(细胞质动力蛋白-1)是一种多亚基运动蛋白复合物,参与细胞内运输和有丝分裂。动力蛋白LIC(轻中间链)亚基LIC1(DLIC-1,基因符号DYNC1LI1)和LIC2(DLIC-2,基因符号DYNC1LI2)与动力蛋白HC(重链)相互排斥,从而定义了不同的功能性CD-1复合物。在这里,我们分析了LIC1和LIC2的有丝分裂分布。我们发现从中期到后期,LIC1定位于有丝分裂纺锤体并在胞质分裂的脱落步骤中集中在中体内。相反,从前期到末期,LIC2强烈定位于主轴极。这些数据表明细胞分裂过程中含LIC1和LIC2的CD-1复合物具有不同的功能。

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