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首页> 外文期刊>Cell biology international. >Effect of actin cytoskeleton disruption on electric pulse-induced apoptosis and electroporation in tumour cells
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Effect of actin cytoskeleton disruption on electric pulse-induced apoptosis and electroporation in tumour cells

机译：肌动蛋白细胞骨架破坏对电脉冲诱导的肿瘤细胞凋亡和电穿孔的影响

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Electric pulses are known to affect the outer membrane and intracellular structures of tumour cells. By applying electrical pulses of 450 ns duration with electric field intensity of 8 kV/cm to HepG2 cells for 30 s, electric pulse-induced changes in the integrity of the plasma membrane, apoptosis, viability and mitochondrial transmembrane potential were investigated. Results demonstrated that electric pulses induced cell apoptosis and necrosis accompanied with the decrease of mitochondrial transmembrane potential and the formation of pores in the membrane. The role of cytoskeleton in cellular response to electric pulses was investigated. We found that the apoptotic and necrosis percentages of cells in response to electric pulses decreased after cytoskeletal disruption. The electroporation of cell was not affected by cytoskeletal disruption. The results suggest that the disruption of actin skeleton is positive in protecting cells from killing by electric pulses, and the skeleton is not involved in the electroporation directly.
机译：已知电脉冲会影响肿瘤细胞的外膜和细胞内结构。通过在HepG2细胞上施加持续时间为450 ns，电场强度为8 kV / cm的电脉冲持续30 s，研究了电脉冲引起的质膜完整性，细胞凋亡，生存力和线粒体跨膜电位的变化。结果表明，电脉冲诱导细胞凋亡和坏死，伴随着线粒体跨膜电位的降低和膜孔的形成。研究了细胞骨架在细胞对电脉冲反应中的作用。我们发现细胞骨架破裂后，细胞对电脉冲的凋亡和坏死百分比降低。细胞的电穿孔不受细胞骨架破坏的影响。结果表明，肌动蛋白骨架的破坏在保护细胞免受电脉冲杀死方面是积极的，并且骨架不直接参与电穿孔。

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