Enhanced expression of MYF5 and MYOD1 in fibroblast cells via the forced expression of bos taurus MYF5

NieY.W.; DingX.B.; GeX.G.; FanH.L.; LiuZ.; GuoH.

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Enhanced expression of MYF5 and MYOD1 in fibroblast cells via the forced expression of bos taurus MYF5

机译：通过金牛座MYF5的强制表达增强成纤维细胞中MYF5和MYOD1的表达

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The formation of vertebrate skeletal muscles widely thought to be under the control of hierarchy of regulatory genes. MYF5 is one of the myogenic determination gene expressed in the developing mouse dermomyotome which control skeletal muscle differentiation. In the current work, we had obtained the cDNA sequence including the full coding region of the bos taurus myogenic factor MYF5 by reverse transcription polymerase chain reaction. Furthermore, we examined whether fibroblast cell derived from mouse and bos taurus can be transduced using plasmid vectors carrying bos taurus MYF5. Bos taurus MYF5 activates MYF5 and MYOD1 expression after 1 day culture. The concerted upregulation of the myogenic regulatory factors enhanced myosin (skeletal fast) expression. These observation show that MYF5 is essential for myogenic differentiation and provides candidates for regulation bos taurus skeletal muscle development.

机译：人们普遍认为脊椎动物骨骼肌的形成受调节基因的等级控制。 MYF5是在发育中的小鼠皮肌球蛋白表达中表达的肌源性测定基因之一，它控制骨骼肌的分化。在目前的工作中，我们已经通过逆转录聚合酶链反应获得了包括金牛座肌原性因子MYF5完整编码区的cDNA序列。此外，我们检查了是否可以使用携带金牛座MYF5的质粒载体转导源自小鼠和金牛座的成纤维细胞。金牛座MYF5在培养1天后激活MYF5和MYOD1表达。肌调节因子的协同上调增强了肌球蛋白（骨骼肌快）的表达。这些观察结果表明，MYF5对于成肌分化是必不可少的，并为调节金牛座骨骼肌的发育提供了候选者。

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《Cell biology international.》 |2013年第9期|共5页
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NieY.W.; DingX.B.; GeX.G.; FanH.L.; LiuZ.; GuoH.;
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正文语种 eng
中图分类细胞生物学;
关键词
Bos taurus MYF5; Fibroblast cell transduced; Muscle differentiation;

机译：金牛座MYF5;成纤维细胞转导;肌肉分化;

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1. Enhanced expression of MYF5 and MYOD1 in fibroblast cells via the forced expression of bos taurus MYF5 [J] . NieY.W., DingX.B., GeX.G., Cell biology international. . 2013,第9期

机译：通过金牛座MYF5的强制表达增强成纤维细胞中MYF5和MYOD1的表达
2. Enhanced expression of myogenic differentiation factors and skeletal muscle proteins in human amnion-derived cells via the forced expression of MYOD1 [J] . AkizawaY., KannoH., KawamichiY., Brain & Development . 2013,第4期

机译：通过强迫表达MYOD1增强羊膜来源细胞中肌源性分化因子和骨骼肌蛋白的表达
3. An enhancer directs differential expression of the linked Mrf4 and Myf5 myogenic regulatory genes in the mouse [J] . Chang THT, Primig M, Hadchouel J, Developmental biology . 2004,第2期

机译：增强子指导小鼠中链接的Mrf4和Myf5肌源性调节基因的差异表达
4. Cancer-associated fibroblast expression of serine protease fibroblast activation protein increases tumour metastatic potential and alters cell death mechanisms [C] . Baird S.K., Rentier C., Smyth F.E Incorporating of the Australian Society for Biochemistry and Molecular Biology Combio . 2009

机译：丝氨酸蛋白酶成纤维细胞活化蛋白的癌症相关的成纤维细胞表达增加了肿瘤转移性潜力和改变细胞死亡机制
5. Regulation of epaxial myogenesis and Myf5 expression by Shh signaling, Gli activation, and BMP antagonism. [D] . Gustafsson, Marcus. 2006

机译：通过Shh信号传导，Gli激活和BMP拮抗作用调节外轴肌发生和Myf5表达。
6. Expression of Cd34 and Myf5 Defines the Majority of Quiescent Adult Skeletal Muscle Satellite Cells [O] . Jonathan R. Beauchamp, Louise Heslop, David S.W. Yu, 2000

机译：Cd34和Myf5的表达定义了大多数静止的成年骨骼肌卫星细胞
7. The A17 enhancer directs expression of Myf5 to muscle satellite cells but Mrf4 to myonuclei [O] . Chang, T H T, Vincent, S D, Buckingham, M E, 2007

机译：A17增强子可将Myf5的表达引导至肌肉卫星细胞，而将Mrf4的表达引导至肌核

Enhanced expression of MYF5 and MYOD1 in fibroblast cells via the forced expression of bos taurus MYF5

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