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首页> 外文期刊>Cell and Tissue Research >How the concentration of insulin affects the development of preantral follicles in goats.
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How the concentration of insulin affects the development of preantral follicles in goats.

机译：胰岛素的浓度如何影响山羊前壁卵泡的发育。

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This study investigated the effect of adding different insulin concentrations to the culture medium for goat preantral follicle development in vitro. The ovarian fragments were immediately fixed or cultured for 7 days in MEM with insulin (0, 5, 10 ng/ml and 5 or 10 mug/ml). The results showed that, after 7 days of culture, insulin at 10 ng/ml was the best concentration to preserve follicular viability and ultrastructure, resulting in the highest rates of normal follicles. After 7 days, only treatments with 10 ng/ml and 5 mug/ml of insulin increased follicular activation when compared to other concentrations. Regarding follicular and oocyte growth, the presence of 10 ng/ml of insulin promoted a larger diameter than other treatments. In conclusion, this study shows that addition of 10 ng/ml of insulin to the culture medium improved the survival and stimulated growth of goat preantral follicles.
机译：这项研究调查了在培养基中添加不同胰岛素浓度对体外山羊前腔卵泡发育的影响。立即将卵巢片段固定或在含胰岛素（0、5、10 ng / ml和5或10杯/ ml）的MEM中培养7天。结果显示，在培养7天后，以10 ng / ml的胰岛素保持卵泡活力和超微结构的最佳浓度，从而导致正常卵泡的产生率最高。 7天后，与其他浓度的胰岛素相比，仅用10 ng / ml和5杯/ ml的胰岛素治疗可增加卵泡活化。关于卵泡和卵母细胞的生长，10 ng / ml胰岛素的存在促进了比其他治疗方法更大的直径。总之，这项研究表明，向培养基中添加10 ng / ml胰岛素可以改善山羊腹腔前卵泡的存活率并促进其生长。

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