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首页> 外文期刊>Cell and Tissue Research >Proton receptor GPR68 expression in dendritic-cell-like S100β-positive cells of rat anterior pituitary gland: GPR68 induces interleukin-6 gene expression in extracellular acidification.
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Proton receptor GPR68 expression in dendritic-cell-like S100β-positive cells of rat anterior pituitary gland: GPR68 induces interleukin-6 gene expression in extracellular acidification.

机译：大鼠垂体前叶树突状细胞样S100β阳性细胞中质子受体GPR68的表达：GPR68在细胞外酸化中诱导白介素6基因表达。

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摘要

S100β-positive cells, which do not express the classical pituitary hormones, appear to possess multifunctional properties and are assumed to be heterogeneous in the anterior pituitary gland. The presence of several protein markers has shown that S100β-positive cells are composed of populations such as stem/progenitor cells, epithelial cells, astrocytes and dendritic cells. Recently, we succeeded in separating S100β-positive cells into round-cell (dendritic-cell-like) and process-cell types. We also found the characteristic expression of anti-inflammatory factors (interleukin-6, Il-6) and membrane receptors (integrin β-6) in the round type. Here, we further investigate the function of the subpopulation of S100β-positive cells. Since IL-6 is also a paracrine factor that regulates hormone producing-cells, we examine whether a correlation exists among extracellular acid stress, IL-6 and hormone production by using primary cultures of anterior pituitary cells. Dendritic-cell-like S100β-positive cells notably expressed Gpr68 (proton receptor) and Il-6. Furthermore, the expression of Il-6 and proopiomelanocortin (Pomc) was up-regulated by extracellular acidification. The functional role of IL-6 and GPR68 in the gene expression of Pomc during extracellular acidification was also examined. Small interfering RNA for Il-6 up-regulated Pomc expression and that for Gpr68 reversed the down-regulation of Il-6 and up-regulated Pomc expression by extracellular acidification. Thus, S100β-positive dendritic-like cells can sense an increase in extracellular protons via GPR68 and respond by the production of IL-6 in order to suppress the up-regulation of Pomc expression.
机译：不表达经典垂体激素的S100β阳性细胞似乎具有多功能特性，并被认为在垂体前叶中是异质的。几种蛋白质标记物的存在已经表明S100β阳性细胞由诸如干/祖细胞，上皮细胞，星形胶质细胞和树突状细胞的群体组成。最近，我们成功地将S100β阳性细胞分为圆形细胞（树突状细胞样）和过程细胞类型。我们还发现了圆形的抗炎因子（白介素6，II-6）和膜受体（整合素β-6）的特征性表达。在这里，我们进一步研究S100β阳性细胞亚群的功能。由于IL-6也是调节激素产生细胞的旁分泌因子，因此我们通过使用垂体前叶细胞原代培养检查细胞外酸应激，IL-6和激素产生之间是否存在相关性。树突状细胞样S100β阳性细胞特别表达Gpr68（质子受体）和Il-6。此外，通过细胞外酸化上调了II-6和proopiomelanocortin（Pomc）的表达。还检查了IL-6和GPR68在细胞外酸化过程中Pomc基因表达中的功能作用。通过细胞外酸化作用，Il-6上调的小干扰RNA和Gpr68的小干扰RNA逆转了Il-6的下调和Pomc表达的上调。因此，S100β阳性树突状细胞可以通过GPR68感知细胞外质子的增加，并通过IL-6的产生做出反应，从而抑制Pomc表达的上调。

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