...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Cellular response to orthodontically-induced short-term hypoxia in dental pulp cells
【24h】

Cellular response to orthodontically-induced short-term hypoxia in dental pulp cells

机译：牙髓细胞对正畸引起的短期缺氧的细胞反应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Orthodontic force application is well known to induce sterile inflammation, which is initially caused by the compression of blood vessels in tooth-supporting apparatus. The reaction of periodontal ligament cells to mechanical loading has been thoroughly investigated, whereas knowledge on tissue reactions of the dental pulp is rather limited. The aim of the present trial is to analyze the effect of orthodontic treatment on the induction and cellular regulation of intra-pulpal hypoxia. To investigate the effect of orthodontic force on dental pulp cells, which results in circulatory disturbances within the dental pulp, we used a rat model for the immunohistochemical analysis of the accumulation of hypoxia-inducible factor-1α in the initial phase of orthodontic tooth movement. To further examine the regulatory role of circulatory disturbances and hypoxic conditions, we analyze isolated dental pulp cells from human teeth with regard to their specific reaction under hypoxic conditions by means of flow cytometry, immunoblot, ELISA and real-time PCR on markers (Hif-1α, VEGF, Cox-2, IL-6, IL-8, ROS, p65). In vivo experiments showed the induction of hypoxia in dental pulp after orthodontic tooth movement. The induction of oxidative stress in human dental pulp cells showed up-regulation of the pro-inflammatory and angiogenic genes Cox-2, VEGF, IL-6 and IL-8. The present data suggest that orthodontic tooth movement affects dental pulp circulation by hypoxia, which leads to an inflammatory response inside treated teeth. Therefore, pulp tissue may be expected to undergo a remodeling process after tooth movement.
机译：众所周知，施加正畸力会引起无菌炎症，该炎症最初是由牙齿支撑装置中血管的压缩引起的。牙周膜细胞对机械负荷的反应已被彻底研究，而有关牙髓组织反应的知识却相当有限。本试验的目的是分析正畸治疗对髓内缺氧的诱导和细胞调节的影响。为了研究正畸力对牙髓细胞的影响，从而导致牙髓内部的循环紊乱，我们使用了大鼠模型对正畸牙齿运动初期缺氧诱导因子-1α的积累进行了免疫组织化学分析。为了进一步检查循环系统紊乱和低氧条件的调节作用，我们通过流式细胞仪，免疫印迹，ELISA和实时荧光定量PCR（Hif- 1α，VEGF，Cox-2，IL-6，IL-8，ROS，p65）。体内实验显示了正畸牙齿移动后牙髓缺氧的诱导。人牙髓细胞中氧化应激的诱导显示促炎和血管生成基因Cox-2，VEGF，IL-6和IL-8的上调。目前的数据表明，正畸牙齿运动通过缺氧影响牙髓的循环，这导致了治疗牙齿内部的炎症反应。因此，可以期望牙髓组织在牙齿运动后经历重塑过程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号