...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Regulation of OPG and RANKL expressed by human dental follicle cells in osteoclastogenesis
【24h】

Regulation of OPG and RANKL expressed by human dental follicle cells in osteoclastogenesis

机译：人牙囊细胞表达OPG和RANKL在破骨细胞形成中的调控

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigate whether the expression of the receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) in human dental follicle cells (HDFCs) regulated by colony stimulating factor 1 (CSF-1), parathyroid hormone-related protein (PTHrP) and bone morphogenetic protein-2 (BMP-2) contributes to osteoclastogenesis. Adolescent human impacted third mandibular molars were used to separate HDFCs. These cells were incubated with PTHrP (10 ng/ml), CSF-1 (25 ng/ml), or BMP-2 (100 ng/ml) for 0.5, 1, 3, 6 and 12 h. The expression of OPG and RANKL was investigated by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Two co-culture systems and tartrate-resistant acid phosphatase (TRAP) staining were used to examine osteoclast formation. Scanning electron microscopy was utilized for the resorption pit assay. RANKL and OPG were expressed innately in HDFCs. Exogenous PTHrP, CSF-1 and BMP-2 chronologically regulated the expression of RANKL and OPG in HDFCs. PTHrP and CSF-1 had similar regulative patterns leading to the up-regulated expression of RANKL and the down-regulated expression of OPG and opposite for BMP-2. The number of TRAP-positive peripheral blood mononuclear cells (PBMCs) slightly increased in contacted co-culture of HDFCs and PBMCs, whereas secreted OPG from HDFCs inhibited osteoclastogenesis in the transwell co-culture system. Contacted co-culture of HDFCs and PBMCs exhibited small and shallow resorption pits, whereas in the transwell co-culture system, secreted OPG from HDFCs reduced the resorption pits, reflecting the difference in osteoclast production. Collectively, we found a dual action of HDFCs in osteoclastogenesis; moreover, PTHrP, CSF-1 and BMP-2 might influence osteoclastogenesis by regulating the expression of RANKL and OPG in HDFCs.
机译：我们调查是否由集落刺激因子1（CSF-1），甲状旁腺激素相关蛋白（ PTHrP）和骨形态发生蛋白2（BMP-2）有助于破骨细胞生成。受青春期影响的下颌第三磨牙用于分离HDFC。将这些细胞与PTHrP（10 ng / ml），CSF-1（25 ng / ml）或BMP-2（100 ng / ml）孵育0.5、1、3、6和12 h。通过免疫组织化学和逆转录聚合酶链反应（RT-PCR）研究OPG和RANKL的表达。两种共培养系统和抗酒石酸酸性磷酸酶（TRAP）染色用于检查破骨细胞的形成。扫描电子显微镜用于吸收坑测定。 RANKL和OPG在HDFC中先天表达。外源性PTHrP，CSF-1和BMP-2按时间顺序调节HDFC中RANKL和OPG的表达。 PTHrP和CSF-1具有相似的调控模式，导致RANKL的表达上调和OPG的表达下调，而BMP-2则相反。在HDFC和PBMC的接触式共培养中，TRAP阳性外周血单个核细胞（PBMC）的数量略有增加，而HDFC分泌的OPG抑制了跨孔共培养系统中的破骨细胞生成。 HDFC和PBMC的接触式共培养显示出小的和浅的吸收坑，而在Transwell共培养系统中，HDFC分泌的OPG减少了吸收坑，反映了破骨细胞生产的差异。总的来说，我们发现HDFC在破骨细胞形成中具有双重作用。此外，PTHrP，CSF-1和BMP-2可能通过调节HDFCs中RANKL和OPG的表达来影响破骨细胞的形成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号