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首页> 外文期刊>Cell and Tissue Research >Scaffolding protein GOPC regulates tight junction structure
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Scaffolding protein GOPC regulates tight junction structure

机译：支架蛋白GOPC调节紧密连接结构

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摘要

GOPC (FIG/PIST/CAL) is a PDZ-domain scaffolding protein that regulates the trafficking of a wide array of proteins, including small GTPases, receptors and cell surface molecules such as cadherin 23 and cystic fibrosis transmembrane regulator. In Madin-Darby canine kidney (MDCK) cells, we find that GOPC localizes to the trans-Golgi network (TGN) but not to the cis-or trans-Golgi cisternae. Colocalization occurs with the early endosome Rab GTPase Rab5 and a TGN/endosome marker Rab14 but not with Rab11, a marker of recycling endosomes. No localization of GOPC was detected to the lateral membranes or tight junctions. Knockdown of GOPC in MDCK cells results in decreased transepithelial resistance and increased paracellular flux. This might be attributable to the compromised trafficking of tight junction components from the TGN, as GOPC-knockdown cells have decreased lateral labeling of the tight junction protein claudin-1 and decreased protein levels of claudin-2. GOPC might mediate the trafficking of newly synthesized tight junction proteins from the TGN to the cell surface or the recycling of these proteins from specialized endosomal compartments.
机译：GOPC（FIG / PIST / CAL）是一种PDZ结构域支架蛋白，可调节多种蛋白的运输，包括小GTPase，受体和细胞表面分子，如钙粘蛋白23和囊性纤维化跨膜调节剂。在Madin-Darby犬肾（MDCK）细胞中，我们发现GOPC定位于反式高尔基体（TGN），而不是顺式或反式高尔基体池。与早期内体Rab GTPase Rab5和TGN /内体标记Rab14共定位，但与Rab11（回收内体的标记）不共定位。没有检测到GOPC定位于侧膜或紧密连接。击倒MDCK细胞中的GOPC导致跨上皮抵抗力降低和细胞旁通量增加。这可能归因于来自TGN的紧密连接成分的运输受到损害，因为GOPC组合式细胞的紧密连接蛋白claudin-1的侧向标记减少，claudin-2的蛋白质水平降低。 GOPC可能介导新合成的紧密连接蛋白从TGN到细胞表面的运输或这些蛋白从专门的内体区室的回收。

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