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首页> 外文期刊>Cell biology international. >Farnesyl diphosphate synthase activity affects ergosterol level and proliferation of yeast Saccharomyces cerevisae.
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Farnesyl diphosphate synthase activity affects ergosterol level and proliferation of yeast Saccharomyces cerevisae.

机译：法呢基二磷酸合酶活性会影响麦角固醇水平和酵母酿酒酵母的增殖。

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摘要

The yeast farnesyl diphosphate synthase (FPPS) gene was engineered so as to construct allelic forms giving various activities of the enzyme. One of the substitutions was F96W in the chain length determination region. The other, K197, conserved within a consensus sequence found in the majority of FPP and GGPP synthases, was substituted by R, E and V. An intricate correlation has been found between the FPPS activity, the amount of ergosterol synthesized and cell growth of a mutant strain defective in FPPS. About 40% of wt FPPS activity was sufficient to support normal growth of the mutant. With further decline of FPPS activity (20 down to 3%) the amount of ergosterol remained unchanged at approximately 0.16% (vs dry weight), whereas growth yield decreased and lag times increased. We postulate that, in addition to ergosterol initiating and maintaining growth of yeast cells, FPP and/or its derivatives participate in these processes.
机译：对酵母法呢基二磷酸合酶（FPPS）基因进行了工程改造，以构建赋予该酶多种活性的等位基因形式。取代之一是在链长确定区域中的F96W。另一个，K197，在大多数FPP和GGPP合成酶的共有序列中保守，被R，E和V取代。已发现FPPS活性，合成的麦角固醇的量和AFP的细胞生长之间存在复杂的关系。 FPPS有缺陷的突变菌株。 wt FPPS活性的约40％足以支持突变体的正常生长。随着FPPS活性的进一步下降（20下降至3％），麦角固醇的量保持不变，约为0.16％（相对于干重），而生长产量下降且滞后时间增加。我们假设，除了麦角固醇引发和维持酵母细胞的生长外，FPP和/或其衍生物也参与了这些过程。

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