Comparison of the effect of hormones on the hormone synthesis of Tetrahymena in medium or salt solution.

Csaba G; Lajko E; Pallinger E

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Comparison of the effect of hormones on the hormone synthesis of Tetrahymena in medium or salt solution.

机译：在中等或盐溶液中激素对四膜虫激素合成的影响比较。

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Tetrahymena pyriformis was maintained in TYM (tryptone-yeast medium) as well as in Losina salt solution. One hour treatment of 10(1) M histamine, serotonin or insulin was given before the histamine, serotonin, triiodothyronine and adrenocorticotropin contents of the cells were measured by flow cytometry after immunocytochemical staining. Maintenance in salt solution increased the hormone level in the cells, and use of the treatment hormone treatments further increased the endogenous hormone content relative to that in medium. The cells in salt mimic better the natural conditions, which means that the effects of hormones under more natural conditions are expressed to a greater extent than the exogenously given hormones in TYM typically used under laboratory conditions. Intercellular hormonal communication between the cells of a Tetrahymena population might assist in the survival of the individual cells.

机译：在TYM（色氨酸-酵母培养基）以及Losina盐溶液中维持梨形四膜虫。在免疫细胞化学染色后，通过流式细胞术测量细胞中的组胺，血清素，三碘甲状腺素和肾上腺皮质激素含量，然后给予10（1）M组胺，血清素或胰岛素一小时。维持盐溶液可增加细胞中的激素水平，并且使用激素处理方法相对于培养基中的内源激素含量进一步增加。盐中的细胞可以更好地模拟自然条件，这意味着与在实验室条件下通常使用的TYM中外源给予的激素相比，在更自然条件下激素的作用表达程度更高。四膜虫种群细胞之间的细胞内激素通讯可能有助于单个细胞的存活。

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《Cell biology international.》 |2010年第11期|共4页
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Csaba G; Lajko E; Pallinger E;
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中图分类细胞生物学;
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1. Comparison of the effect of hormones on the hormone synthesis of Tetrahymena in medium or salt solution. [J] . Csaba G, Lajko E, Pallinger E Cell biology international. . 2010,第11期

机译：在中等或盐溶液中激素对四膜虫激素合成的影响比较。
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Comparison of the effect of hormones on the hormone synthesis of Tetrahymena in medium or salt solution.

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