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首页> 外文期刊>Cell biology international. >Sphere-forming cells from peripheral cornea demonstrate polarity and directed cell migration
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Sphere-forming cells from peripheral cornea demonstrate polarity and directed cell migration

机译：来自周围角膜的球形细胞显示极性和定向细胞迁移

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Sphere-forming cells from peripheral cornea represent a potential source of progenitor cells for treatment of corneal degenerative diseases. Control of cellular repopulation on transplantable substrates is important to prevent uncontrolled growth in unfavourable directions. The coordination of cellular outgrowth may be in response to environmental cues and/or cellular signals from other spheres. To investigate this, cell migration patterns were observed following placement of spheres on an adhesive surface. Human peripheral corneal cells were maintained using a sphere-forming assay and their behaviour on collagen substrate recorded by time-lapse imaging. Immunocytochemistry and proliferation assays were used to detect protein expression and cell division. Proliferation assays showed that spheres formed by a combination of cell division and aggregation. Cell division continued within spheres for up to 4 months and was up-regulated when exposed to differentiation medium and collagen substrate. The spheres expressed both epithelial and stromal cell markers. When exposed to collagen; (1) 25% of the spheres showed spontaneous polarised outgrowth. (2) One sphere initially showed polarised outgrowth followed by collective migration with discrete morphological changes to form leading and trailing compartments. (3) A sphere which did not show polarised outgrowth was also capable of collective migration using cell protrusion and retraction. (4) Active recruitment of cells into spheres was observed. (5) Placement of spheres in close proximity led to production of a cell exclusion area adjacent to spheres. Thus peripheral corneal cell spheres are dynamic entities capable of developing polarity and modifying migration in response to their environment.
机译：来自周围角膜的形成球的细胞代表了用于治疗角膜变性疾病的祖细胞的潜在来源。控制可移植基质上细胞的繁殖对于防止在不利的方向上不受控制的生长很重要。细胞生长的协调可以响应于环境提示和/或来自其他领域的细胞信号。为了对此进行研究，在将球放置在粘合剂表面上之后观察到细胞迁移模式。使用球形形成测定法维持人外周角膜细胞，并通过延时成像记录它们在胶原基质上的行为。免疫细胞化学和增殖测定法用于检测蛋白质表达和细胞分裂。增殖试验表明，球体是由细胞分裂和聚集共同形成的。细胞分裂在球体内持续进行长达4个月，当暴露于分化培养基和胶原底物时细胞分裂被上调。球体表达上皮和基质细胞标记。接触胶原蛋白时; （1）25％的球体显示出自发极化的生长。 （2）一个球体最初显示出极化的生长，然后集体迁移，形态上离散，形成前室和后室。 （3）没有极化增长的球体也能够通过细胞的伸出和缩回而集体迁移。 （4）观察到细胞主动募集到球中。 （5）将球紧密放置会导致在球附近产生细胞排斥区域。因此，外周角膜细胞球是能够发展极性并响应于其环境而改变迁移的动态实体。

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