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首页> 外文期刊>Letters in Applied Microbiology >Screening of food raw materials for the presence of Shiga toxin-producing Escherichia coli O91:H21
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Screening of food raw materials for the presence of Shiga toxin-producing Escherichia coli O91:H21

机译：筛选产生志贺毒素的大肠杆菌O91：H21的食品原料

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AIMS: To provide information on the prevalence and detection, in foods, of Shiga toxin-producing Escherichia coli (STEC) O91:H21. METHODS AND RESULTS: Seven hundred fifteen minced beef meats and 205 raw milk samples were analysed by stx-specific PCR-ELISA. Samples positive for stx were subsequently tested for the presence of wzy-O91, fliC-H21 and the adhesin-encoding gene saa. For minced meat, 16 (2.2%) and 11 (1.5%) samples were found positive for (stx, wzy-O91, fliC-H21) and (stx, wzy-O91, fliC-H21, saa) combinations, respectively. For raw milk, seven (3.4%) samples were found positive for the (stx, wzy-O91, fliC-H21) combination but none of these contained saa. Two STEC O91:H21 saa-positive strains and three STEC O91 H21- and saa-negative strains were isolated by colony hybridization. CONCLUSIONS: A low prevalence of potentially pathogenic STEC O91:H21 in food products was found using a combination of PCR assays targeting stx, wzy-O91, fliC-H21 and saa. SIGNIFICANCE AND IMPACT OF THE STUDY: The PCR-based approach described here represents a valuable method for rapid screening of food samples contaminated by STEC O91:H21.
机译：目的：提供有关食品中志贺毒素生产大肠埃希菌O91：H21的流行和检测的信息。方法与结果：采用stx特异的PCR-ELISA法对515份切碎的牛肉和205份生乳样品进行了分析。随后测试对stx呈阳性的样品中是否存在wzy-O91，fliC-H21和粘附素编码基因saa。对于碎肉，分别发现（stx，wzy-O91，fliC-H21）和（stx，wzy-O91，fliC-H21，saa）组合为阳性的样品（16％（2.2％）和11％（1.5％））。对于原料奶，发现有七个（3.4％）样品的（stx，wzy-O91，fliC-H21）组合呈阳性，但这些样品都不包含saa。通过菌落杂交分离了两个STEC O91：H21 saa阳性菌株以及三个STEC O91 H21和saa阴性菌株。结论：结合针对stx，wzy-O91，fliC-H21和saa的PCR检测，发现食品中潜在致病性STEC O91：H21的患病率较低。研究的意义和影响：此处描述的基于PCR的方法代表了一种有价值的方法，用于快速筛查受STEC O91：H21污染的食物样品。

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