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首页> 外文期刊>Letters in Applied Microbiology >Comparison of eleven commercially available rapid tests for detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis
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Comparison of eleven commercially available rapid tests for detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis

机译：十一种商业快速检测炭疽芽孢杆菌，土拉弗朗西斯菌和鼠疫耶尔森菌的快速检测方法的比较

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摘要

Yersinia pestis, Bacillus anthracis and Francisella tularensis cause serious zoonotic diseases and have the potential to cause high morbidity and mortality in humans. In case of natural outbreaks and deliberate or accidental release of these pathogens rapid detection of the bacteria is crucial for limitation of negative effects of the release. In the present study, we evaluated 11 commercially available rapid test kits for the detection of Y.pestis, B.anthracis and F.tularensis in terms of sensitivity, specificity and simplicity of the procedure. The results revealed that rapid and easy-to-perform lateral flow assays for detection of highly pathogenic bacteria have very limited sensitivity. In contrast, the immunofiltration assays showed high sensitivity but limited specificity and required a too complicated procedure to be applied in the field by nonlaboratory workers (e.g. First Responders like fire, police and emergency medical personnel). Each sample - whether tested negative or positive by the rapid tests - should be retested in a reference laboratory using validated methods.
机译：鼠疫耶尔森氏菌，炭疽芽孢杆菌和土拉弗朗西斯菌引起严重的人畜共患疾病，并有可能导致人类高发病率和高死亡率。在自然爆发以及故意或意外释放这些病原体的情况下，细菌的快速检测对于限制释放的负面影响至关重要。在本研究中，我们评估了11种可商购的快速检测试剂盒，它们在敏感性，特异性和操作简便性方面均能检测pestis，B.anthracis和F.tularensis。结果表明，用于检测高致病性细菌的快速且易于执行的侧向流动检测的灵敏度非常有限。相反，免疫过滤测定法显示出高灵敏度但特异性有限，并且要求非实验室工作人员（例如消防，警察和急救人员等急救人员）在现场应用过于复杂的程序。每个样品（无论是通过快速测试检测为阴性还是阳性）都应在参考实验室中使用经过验证的方法进行重新测试。

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