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首页> 外文期刊>Cell biology international. >In vitro effect of biogenic amines on the hormone content of immune cells of the peritoneal fluid and thymus. Is there a hormonal network inside the immune system?
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In vitro effect of biogenic amines on the hormone content of immune cells of the peritoneal fluid and thymus. Is there a hormonal network inside the immune system?

机译：生物胺对腹膜液和胸腺免疫细胞激素含量的体外影响。免疫系统内部是否存在激素网络？

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摘要

Immune cells contain different hormones and hormone-like molecules, such as insulin, endorphin, triiodothyronine (T3) histamine, serotonin. In earlier in vitro experiments insulin down-regulated histamine, serotonin and T3 content of thymus cells. Now we studied the effect of biogenic amines on the endorphin, T3, serotonin and histamine content of rat peritoneal and thymic cells. Cells were obtained from male rats of 100g body weight. 100 ng/ml serotonin or 300 ng/ml histamine was added for 30 min. After that the cells were prepared for flow cytometric analysis with antibodies to endorphin, T3, histamine and serotonin as primary antibodies and anti-rabbit IgG as secondary antibody. Finishing the measurements the cells were also studied by confocal microscopy. T3 concentration (binding of anti-T3 antibody) increased in peritoneal mast cells after serotonin treatment and in the monocyte-macrophage-granulocyte group after histamine treatment. Thymocytes' T3 content radically decreased after both treatments. Serotonin and histamine treatment also radically reduced the amine content of each other. Endorphin level was resistant to hormonal treatments. The results call attention to a possible hormonal network inside the immune system in which hormones produced by the immune cells themselves can influence each other.
机译：免疫细胞包含不同的激素和类激素分子，例如胰岛素，内啡肽，三碘甲状腺素（T3）组胺，血清素。在较早的体外实验中，胰岛素下调了胸腺细胞的组胺，血清素和T3含量。现在我们研究了生物胺对大鼠腹膜和胸腺细胞内啡肽，T3，血清素和组胺含量的影响。从体重为100g的雄性大鼠中获得细胞。加入30分钟的100ng / ml的5-羟色胺或300ng / ml的组胺。之后，准备细胞用于流式细胞术分析，以抗内啡肽，T3，组胺和血清素的抗体为一抗，抗兔IgG抗体为二抗。完成测量后，还通过共聚焦显微镜研究了细胞。血清素处理后腹膜肥大细胞和组胺处理后单核-巨噬细胞-粒细胞组的T3浓度（抗T3抗体的结合）增加。两种处理后胸腺细胞的T3含量均急剧下降。 5-羟色胺和组胺的治疗也从根本上降低了彼此的胺含量。内啡肽水平对激素治疗有抵抗力。这些结果引起人们对免疫系统内部可能的激素网络的关注，在该网络中免疫细胞自身产生的激素可以相互影响。

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