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首页> 外文期刊>Cell biology international. >Macrophage-associated erythropoiesis and lymphocytopoiesis in mouse fetal liver: ultrastructural and ISH analysis.
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Macrophage-associated erythropoiesis and lymphocytopoiesis in mouse fetal liver: ultrastructural and ISH analysis.

机译：小鼠胎儿肝脏中巨噬细胞相关的红细胞生成和淋巴细胞生成：超微结构和ISH分析。

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摘要

To elucidate the process of fetal liver hematopoiesis, the relationships between stroma and hematopoietic cells involved in maturation were investigated. Cultured mouse fetal liver explants were established for morphological analysis of the interactions between fetal liver stroma and hematopoietic cells ex vivo. Fetal liver stroma comprised epithelial cells and macrophages, which occupied most of the culture surface. Macrophages proliferated extensively in primary culture, but almost disappeared after 3 passages. Close morphological and functional relationships were established between macrophages and hemopoietic cells, whereas epithelial cells did not interact with blood cells. Scanning electron microscopy revealed that macrophages were in close contact with erythroblasts and formed a three-dimensional network. In each erythroblastic island, 2-3 lymphocytes were also in contact with the macrophages; erythroblasts, lymphocytes and macrophages formed close, firm associations through their cytoplasmic membranes. This cell orientation was confirmed by transmission electron microscopy of fetal liver in vivo. In situ hybridization revealed that the macrophages expressed jagged-1, an important ligand of the Notch signaling system in hematopoiesis.
机译：为了阐明胎儿肝脏造血过程，研究了基质与造血细胞参与成熟的关系。建立培养的小鼠胎肝外植体，用于离体分析胎肝基质与造血细胞之间相互作用的形态。胎儿肝基质由上皮细胞和巨噬细胞组成，它们占据了大部分培养表面。巨噬细胞在原代培养物中大量增殖，但经过3代后几乎消失。在巨噬细胞和造血细胞之间建立了紧密的形态和功能关系，而上皮细胞不与血细胞相互作用。扫描电子显微镜显示，巨噬细胞与成红细胞紧密接触并形成三维网络。在每个红细胞生成岛中，还有2-3个淋巴细胞也与巨噬细胞接触。成红细胞，淋巴细胞和巨噬细胞通过其细胞质膜形成紧密而牢固的联系。该细胞定向通过体内胎儿肝的透射电子显微镜确认。原位杂交显示巨噬细胞表达锯齿状1，这是造血过程中Notch信号系统的重要配体。

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