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首页> 外文期刊>Cell biology international. >Vasopressin stimulates tyrosine phosphorylation by activation of PKC in the rat smooth muscle cell line, A-10.
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Vasopressin stimulates tyrosine phosphorylation by activation of PKC in the rat smooth muscle cell line, A-10.

机译：加压素通过激活大鼠平滑肌细胞系A-10中的PKC来刺激酪氨酸磷酸化。

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摘要

Arginine vasopressin (AVP)-induced tyrosine phosphorylation was studied in a rat smooth muscle cell line, A-10, by western blotting, using a monoclonal antibody against phosphotyrosine. AVP stimulated the phosphorylation of several cellular proteins of molecular mass 60-130 kDa in a time- and dose-dependent manner. Phosphorylation was mediated largely by V(1)receptor subtype since it was inhibited by selective V(1)antagonist and was only partially elicited by the V(2)agonist, desmopressin. Heterotrimeric G-proteins seemed to be involved in the phosphorylation mechanism because fluoraluminates, an activator of heterotrimeric G-proteins (and thus an uncoupler of the receptor-G-protein interaction) inhibited the AVP-induced phosphorylation. The protein kinase C (PKC) inhibitors: staurosporine, H7 and GF109203X are able to block the AVP-stimulated phosphorylation. The last of these has been shown to be one of the most selective inhibitors of PKC. These results indicate that PKC is upstream of the phosphorylation, a motion which is supported by the fact that the AVP-stimulated phosphorylation was downregulated by phorbol esters. Copyright 1999 Academic Press.
机译：使用抗磷酸酪氨酸的单克隆抗体，通过蛋白质印迹法在大鼠平滑肌细胞系A-10中研究了精氨酸加压素（AVP）诱导的酪氨酸磷酸化。 AVP以时间和剂量依赖性方式刺激了分子质量为60-130 kDa的几种细胞蛋白的磷酸化。磷酸化主要由V（1）受体亚型介导，因为它被选择性V（1）拮抗剂抑制，而仅由V（2）激动剂去氨加压素部分诱导。异三聚体G蛋白似乎参与了磷酸化机制，因为异三聚体G蛋白的激活剂氟铝酸盐（因而受体G蛋白相互作用的解偶联剂）会抑制AVP诱导的磷酸化。蛋白激酶C（PKC）抑制剂：星形孢菌素，H7和GF109203X能够阻断AVP刺激的磷酸化。这些中的最后一种已被证明是最有选择性的PKC抑制剂之一。这些结果表明PKC在磷酸化的上游，该运动受到佛波酯下调AVP刺激的磷酸化的事实的支持。版权所有1999，学术出版社。

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