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首页> 外文期刊>Cell biology international. >Salidroside induces rat mesenchymal stem cells to differentiate into dopaminergic neurons.
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Salidroside induces rat mesenchymal stem cells to differentiate into dopaminergic neurons.

机译：红景天苷诱导大鼠间充质干细胞分化为多巴胺能神经元。

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摘要

Parkinson's disease (PD) is a neurodegenerative disorder characterised by the loss of substantia nigra dopaminergic neurons that leads to a reduction in striatal dopamine (DA) levels. Replacing lost cells by transplanting dopaminergic neurons has potential value to repair the damaged brain. Salidroside (SD), a phenylpropanoid glycoside isolated from plant Rhodiola rosea, is neuroprotective. We examined whether salidroside can induce mesenchymal stem cells (MSCs) to differentiate into neuron-like cells, and convert MSCs into dopamine neurons that can be applied in clinical use. Salidroside induced rMSCs to adopt a neuronal morphology, upregulated the expression of neuronal marker molecules, such as gamma neuronal enolase 2 (Eno2/NSE), microtubule-associated protein 2 (Map2), and beta 3 class III tubulin (Tubb3/β-tubulin III). It also increased expression of brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and nerve growth factor (NGF) mRNAs, and promoted the secretion of these growth factors. The expression of dopamine neurons markers, such as dopamine-beta-hydroxy (DBH), dopa decarboxylase (DDC) and tyrosine hydroxylase (TH), was significantly upregulated after treatment with salidroside for 1-12 days. DA steadily increased after treatment with salidroside for 1-6 days. Thus salidroside can induce rMSCs to differentiate into dopaminergic neurons.
机译：帕金森氏病（PD）是一种神经退行性疾病，其特征是黑质多巴胺能神经元丧失，导致纹状体多巴胺（DA）水平降低。通过移植多巴胺能神经元替代丢失的细胞具有修复受损大脑的潜在价值。红景天苷（SD）是一种从植物红景天中分离出来的苯基丙烷类糖苷，具有神经保护作用。我们检查了红景天苷是否可以诱导间充质干细胞（MSCs）分化为神经元样细胞，并将MSCs转化为可用于临床的多巴胺神经元。红景天苷诱导rMSCs呈神经元形态，上调神经元标记分子的表达，例如γ神经元烯醇酶2（Eno2 / NSE），微管相关蛋白2（Map2）和β3III类微管蛋白（Tubb3 /β-tubulin） III）。它还增加了脑源性神经营养因子（BDNF），神经营养蛋白3（NT-3）和神经生长因子（NGF）mRNA的表达，并促进了这些生长因子的分泌。红景天苷处理1-12天后，多巴胺神经元标志物（如多巴胺-β-羟基（DBH），多巴脱羧酶（DDC）和酪氨酸羟化酶（TH））的表达显着上调。红景天苷治疗1-6天后，DA稳定增加。因此，红景天苷可以诱导rMSC分化为多巴胺能神经元。

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