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首页> 外文期刊>Cell biology international. >GALECTIN-3 expression in differentiating human myeloid cells.
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GALECTIN-3 expression in differentiating human myeloid cells.

机译:GALECTIN-3在分化人类骨髓细胞中的表达。

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Galectin-3 is an endogenous mammalian carbohydrate-binding protein with affinity for ABH group carbohydrate epitopes and polylactosamine glycans present on cell surface and extracellular matrix glycoproteins. It has been shown to play a role in cell differentiation, morphogenesis, adhesion and cell proliferation regulation. Progenitor cell proliferation in bone marrow depends on stem cell factors including those modulating their adhesion to the bone marrow stroma. The present study shows that the 32 kD galectin-3 is developmentally expressed in human myeloid cells and is strongly upregulated on the cell surface of late mature myeloid cells. Despite the fact that the expression of the galectin-3 is very low in CD34+ early myeloid cell, a 70 kD protein is found by Western blotting using antibodies specific to galectin-3, exclusively in those cells. Finally, exogenous human recombinant galectin-3 binds strongly to CD34+ early myeloid cells and emphasizes granulocyte-colony stimulating factor (G-CSF) driven proliferation in vitro. Copyright 2000 Academic Press.
机译:Galectin-3是一种内源性哺乳动物碳水化合物结合蛋白,对存在于细胞表面和细胞外基质糖蛋白上的ABH类碳水化合物表位和聚乳糖胺聚糖具有亲和力。已经表明它在细胞分化,形态发生,粘附和细胞增殖调节中起作用。骨髓中祖细胞的增殖取决于干细胞因子,包括那些调节其与骨髓基质粘附的因子。本研究表明,32 kD galectin-3在人类髓样细胞中发育表达,并在晚期成熟髓样细胞的细胞表面上强烈上调。尽管事实证明,galectin-3在CD34 +早期髓样细胞中的表达非常低,但仅使用那些对galectin-3特异的抗体,通过Western blotting发现了一个70 kD的蛋白质。最后,外源人类重组半乳糖凝集素-3与CD34 +早期骨髓细胞牢固结合,并强调粒细胞集落刺激因子(G-CSF)在体外驱动的增殖。版权所有2000学术出版社。

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