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首页> 外文期刊>Cell biology international. >Ganglioside-mediated metabolic synchronization of protein synthesis activity in cultured hepatocytes.
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Ganglioside-mediated metabolic synchronization of protein synthesis activity in cultured hepatocytes.

机译：神经节苷脂介导的肝细胞蛋白质合成活性的代谢同步。

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摘要

An ultradian oscillation of protein synthesis was detected by synchronization of metabolic activity in rat hepatocyte cultures. This oscillation occurs in dense cultures in fresh medium, but not in sparse ones. Metabolic synchronization of sparse cultures, however, was initiated by conditioned medium or addition of 0.3-0.5 microm of a mixture of bovine brain gangliosides to fresh culture medium along with either 0.06-0.2 microm GM1 or 0.1-0.2 microm GDIa. GTIb and GDIb did not produce oscillations, nor did human liver ganglioside GM3. High expression of GM1 ganglioside determinants in hepatocytes maintained in the conditioned medium purified polyclonal antibodies to GM1 was coupled with protein synthetic oscillatory activity, i.e. metabolic synchronization. Incubation of dense cultures with GM1-antibodies for 24 h decreased the amplitude of these oscillations. In sparse cultures maintained in fresh medium where protein synthesis showed no oscillatory pattern, GM1 expression was low. Copyright 2000 Academic Press.
机译：通过大鼠肝细胞培养物中代谢活性的同步检测到蛋白质合成的超循环振荡。这种振荡发生在新鲜培养基中的稠密培养物中，而在稀疏培养基中则没有。稀疏培养物的代谢同步是通过条件培养基或将0.3-0.5微米牛脑神经节苷脂的混合物与0.06-0.2微米GM1或0.1-0.2微米GDIa一起添加到新鲜培养基中而开始的。 GTIb和GDIb不会产生振荡，人肝神经节苷脂GM3也不会产生振荡。在条件培养基中纯化的针对GM1的多克隆抗体中维持的肝细胞中GM1神经节苷脂决定簇的高表达与蛋白质合成的振荡活性（即代谢同步）结合在一起。将密集培养物与GM1抗体一起孵育24小时可降低这些振荡的幅度。在蛋白质合成没有振荡模式的新鲜培养基中维持的稀疏培养中，GM1表达较低。版权所有2000学术出版社。

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