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Protein myozap--a late addition to the molecular ensembles of various kinds of adherens junctions.

机译：蛋白质myozap-各种粘附连接分子合体的新成员。

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摘要

The protein myozap, a polypeptide of 54 kDa, has recently been identified as a component of the cytoplasmic plaques of the composite junctions (areae compositae) in the myocardiac intercalated disks and of the adherens junctions (AJs) in vascular endothelia. Now we report that using very sensitive new antibodies and drastic localization methods, we have also identified this protein as a component of the AJ plaques in simple and complex epithelia, in the adluminal cell layer of the transitional epithelium of the urinary tract and in certain cell layers of diverse stratified epithelia, including gingiva, tongue, pharynx and esophagus, cervix, vagina and epidermis. Myozap has not been identified in desmosomal and tight junction plaques. We have also detected protein myozap in AJ structures of carcinomas. The discovery of a novel major protein in AJ plaques now calls for re-examinations of molecular interactions in AJ formation and maintenance and also offers a new marker for diagnostic immunocytochemistry. We also discuss the need for progressive unravelling, extractive treatments and buffer rinses of sections and cultured cells to reveal obscured or masked antigens, before definitive negative conclusions in immunohistochemistry can be made.
机译：蛋白质myozap，一种54 kDa的多肽，最近已被确定为心肌插层盘中复合连接（areae compositae）和血管内皮粘附连接（AJs）的细胞质斑块的组成部分。现在我们报告说，使用非常敏感的新抗体和严格的定位方法，我们还已将该蛋白鉴定为简单和复杂上皮细胞，尿路过渡上皮的上皮细胞层以及某些细胞中的AJ斑块的成分。分层的上皮层，包括牙龈，舌头，咽和食道，子宫颈，阴道和表皮。在桥粒和紧密连接的斑块中尚未发现Myozap。我们还在癌症的AJ结构中检测到了蛋白myozap。现在，在AJ斑块中发现一种新的主​​要蛋白质需要重新检查AJ形成和维持过程中的分子相互作用，并且还为诊断性免疫细胞化学提供了新的标记。我们还讨论了在进行免疫组织化学确定性否定结论之前，需要进行渐进式拆解，提取处理以及切片和培养细胞的缓冲液漂洗以显示模糊或掩盖的抗原的需要。

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