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首页> 外文期刊>Cell and Tissue Research >Gene expression profiles of FABP genes in protochordates, Ciona intestinalis and Branchiostoma belcheri
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Gene expression profiles of FABP genes in protochordates, Ciona intestinalis and Branchiostoma belcheri

机译：FABP基因在原线虫，Ciona intestinalis和Belcheriostoma belcheri中的基因表达谱

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Fatty-acid-binding proteins (FABPs) are small intracellular proteins associated with the transportation of fatty acids. Members of the FABPs share similar amino acid sequences and tertiary structures and form, together with a member of the cellular retinol-binding proteins (CRBPs), the intracellular-lipid-binding protein (iLBP) family. In vertebrates, several types of FABP have been isolated and classified into three subfamilies: 2-4. In invertebrates, several FABP-related proteins have been reported in protostomes and amphioxus; however, little is known about the relationship between their phylogenetic positions and expression patterns. We have performed a genome-wide survey of FABP-related genes in protochordates: amphioxus Branchiostoma belcheri and the ascidian Ciona intestinalis. Comprehensive BLAST searches in NCBI and the Ciona Ghost Database by using amino acid sequences of all FABPs have revealed that the ascidian C. intestinalis and amphioxus B. belcheri contain six and seven FABP-related genes in their haploid genomes, respectively. Expression pattern analyses by whole-mount in situ hybridization in Ciona transparent juveniles and serial-section in situ hybridizations in adult amphioxus have revealed that all genes are mainly expressed in the postpharyngeal digestive tract. In particular, the expression of FABP-related genes of subfamily-2 (liver/ileum type) and subfamily-3 (intestinal type) in the ascidian pyloric gland and amphioxus hepatic cecum provides insight into the evolution of hepatic-related structures of chordates and FABP-related genes.
机译：脂肪酸结合蛋白（FABP）是与脂肪酸运输相关的小细胞内蛋白。 FABP的成员具有相似的氨基酸序列和三级结构，并与细胞视黄醇结合蛋白（CRBP）的成员一起形成细胞内脂质结合蛋白（iLBP）家族。在脊椎动物中，已分离出几种类型的FABP并将其分为三个亚科：2-4。在无脊椎动物中，有几种FABP相关蛋白已被报道在原虫和双歧杆菌中。但是，关于它们的系统发生位置和表达模式之间的关系知之甚少。我们已经对原线虫中的FABP相关基因进行了全基因组调查：双歧杆菌Belcheri belcheri和海鞘Ciona intestinalis。通过使用所有FABP的氨基酸序列在NCBI和Ciona Ghost数据库中进行的全面BLAST搜索显示，海鞘小肠念珠菌和双歧双歧杆菌分别在其单倍体基因组中包含6个和7个与FABP相关的基因。通过Ciona透明幼虫的全量原位杂交和成年文昌鱼的连续切片原位杂交的表达模式分析表明，所有基因主要在咽后消化道中表达。特别是，在海鞘幽门腺和两栖类肝盲肠中，亚家族2（肝/回肠型）和亚家族3（肠型）的FABP相关基因的表达提供了对与肝有关的脊索和梭状芽胞结构进化的见解。 FABP相关基因。

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