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首页> 外文期刊>Cell and Tissue Research >Effect of LIM kinase 1 overexpression on behaviour of endometriosis-derived stromal cells
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Effect of LIM kinase 1 overexpression on behaviour of endometriosis-derived stromal cells

机译：LIM激酶1过表达对子宫内膜异位症基质细胞行为的影响

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LIM kinase 1 (LIMK1) has been implicated in tumour invasion and migration in several tumour types. However, its role in the progression of endometriosis has not yet been studied. Our aim is to analyse LIMK1 expression in endometriosis-derived stromal cells and to explore the effects of LIMK1 overexpression on their biological behaviour. The mRNA and protein expression levels of LIMK1 of eutopic endometrial stromal cells (ESCs) separated from 30 endometriosis patients and normal endometrial stromal cells (NSCs) separated from 30 patients without endometriosis were analysed by real-time polymerase chain reaction and Western blot. Lentiviral particles containing human LIMK1 short interfering RNA were used to silence the LIMK1 gene in ESCs and LIMK1-expressing lentiviral particles containing the total LIMK1 cDNA was transfected into NSCs to upregulate LIMK1 expression. Cell migration, invasion, proliferation and expression of markers of adhesion, invasion and angiogenesis of ESCs and NSCs were evaluated under basal conditions and after transfection in vitro. The mRNA and protein expression levels of LIMK1 in ESCs were higher than those in NSCs. Under basal conditions, ESCs exhibited greater cell migration, invasion and proliferation and higher levels of markers of adhesion, invasion and angiogenesis than NSCs. The behaviour of ESCs was decreased after LIMK1 gene silencing and that of NSCs was elevated after LIMK1 gene upregulation. Thus, LIMK1 is overexpressed in ESCs thereby facilitating malignant-like behaviour, including enhanced migration, invasion, proliferation and angiogenesis, all of which contribute to the occurrence and development of endometriosis.
机译：LIM激酶1（LIMK1）已牵涉到几种类型的肿瘤的侵袭和迁移。但是，尚未研究其在子宫内膜异位症发展中的作用。我们的目的是分析子宫内膜异位来源的基质细胞中LIMK1的表达，并探讨LIMK1过表达对其生物学行为的影响。通过实时聚合酶链反应和Western blot分析30例子宫内膜异位患者的异位子宫内膜基质细胞（ESCs）和30例非子宫内膜异位患者的正常子宫内膜基质细胞（NSCs）的LIMK1的mRNA和蛋白表达水平。包含人LIMK1短干扰RNA的慢病毒颗粒用于沉默ESC中的LIMK1基因，将包含总LIMK1 cDNA的表达LIMK1的慢病毒颗粒转染到NSC中以上调LIMK1表达。在基础条件下和体外转染后，评估了ESC和NSC的细胞迁移，侵袭，增殖和粘附，侵袭和血管生成的标志物的表达。 ESCs中LIMK1的mRNA和蛋白表达水平高于NSCs。在基础条件下，与NSC相比，ESCs表现出更大的细胞迁移，侵袭和增殖以及更高水平的粘附，侵袭和血管生成标记。 LIMK1基因沉默后，ESCs的行为降低，而LIMK1基因上调后，NSCs的行为升高。因此，LIMK1在ESC中过表达，从而促进恶性行为，包括迁移，侵袭，增殖和血管生成的增强，所有这些都有助于子宫内膜异位症的发生和发展。

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