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首页> 外文期刊>Cell biology international. >EFFECT OF ANTISENSE hTERT mRNA OLIGODEOXYNUCLEOTIDE ON TELOMERASE ACTIVITY OF LEUKEMIC CELLS.
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EFFECT OF ANTISENSE hTERT mRNA OLIGODEOXYNUCLEOTIDE ON TELOMERASE ACTIVITY OF LEUKEMIC CELLS.

机译：反义hTERT mRNA寡聚脱氧核糖核酸对白血病细胞端粒酶活性的影响。

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摘要

The effect of antisense hTERT mRNA oligodeoxynucleotide on telomerase activity of leukemia cells was explored and investigated in the present study. Telomerase activity was measured by the telomerase PCR ELISA assay kit (TRAP); hTERT mRNA expression by reverse transcription polymerase chain reaction (RT-PCR) assay and gel-image system, hTERT protein by immunochemistry and flowcytometry. Results showed Incubation of leukemic cells (HL-60 and K562 cell lines) with 10 micromol/l AS PS-ODN would significantly reduce the their mRNA levels and in vitro expression of hTERT protein 24 h later, so that the telomerase activity would be significantly down-regulated or inhibited. In conlusion, the hTERT AS PS-ODN is an excellent inhibitor for telomerase activity.
机译：本研究探索并研究了反义hTERT mRNA寡脱氧核苷酸对白血病细胞端粒酶活性的影响。端粒酶活性通过端粒酶PCR ELISA测定试剂盒（TRAP）测量。通过逆转录聚合酶链反应（RT-PCR）分析和凝胶图像系统检测hTERT mRNA的表达，通过免疫化学和流式细胞术检测hTERT蛋白。结果显示，将白血病细胞（HL-60和K562细胞系）与10μmol/ l AS PS-ODN一起孵育会在24 h后显着降低其mRNA水平和hTERT蛋白的体外表达，从而使端粒酶活性显着降低。下调或抑制。总之，hTERT AS PS-ODN是端粒酶活性的优异抑制剂。

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