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首页> 外文期刊>Cell biology international. >Proliferating effects of the flavonoids daidzein and quercetin on cultured chicken primordial germ cells through antioxidant action.
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Proliferating effects of the flavonoids daidzein and quercetin on cultured chicken primordial germ cells through antioxidant action.

机译：黄酮类大豆苷元和槲皮素通过抗氧化作用对培养的鸡原始生殖细胞的增殖作用。

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摘要

Primordial germ cells (PGCs) are undifferentiated pluripotent stem cells, whose proliferation is influenced by many internal and external factors. In the present study, a PGC-somatic cell co-culture model was established to evaluate effects of the flavonoids daidzein (DAI) and quercetin (QUE) on proliferation of PGCs from embryonic chickens. PGCs were isolated from the germinal ridge of 3.5-4day embryos and cultured in 5% fetal calf serum (FCS)-supplemented Medium 199. PGC subculture was carried out on chicken embryonic fibroblast feeder (CEF) or follicular granulosa cell feeder (GCF) layers. The subcultured PGCs were challenged with flavonoids alone or in combination with a reactive oxygen substance (ROS)-producing system on CEF for 48h. The results showed a better supporting effect of CEF than GCF. Flavonoids (1microg/ml) significantly promoted PGC proliferation, which could be markedly inhibited by ROS. The oxidative damage by ROS was further manifest by decreased superoxide dismutase activity and glutathione levels. In addition, activation of protein kinase A (PKA) by forskolin significantly stimulated PGC proliferation, but PKA inhibitor H89 inhibited the proliferating effects induced by DAI and QUE. These results indicated that cultured PGCs respond to exogenous agents on proliferation and that antioxidant flavonoids could restore the intracellular antioxidant system and promote PGC proliferation via their antioxidant action involving the PKA signaling pathway.
机译：原始生殖细胞（PGC）是未分化的多能干细胞，其增殖受许多内部和外部因素影响。在本研究中，建立了PGC-体细胞共培养模型以评估黄酮类黄豆苷元（DAI）和槲皮素（QUE）对胚胎鸡PGC增殖的影响。从3.5-4天胚胎的生发脊中分离出PGC，并在添加了5％胎牛血清（FCS）的199培养基中进行培养。PGC在鸡胚成纤维细胞饲养层（CEF）或滤泡颗粒细胞饲养层（GCF）层上进行亚培养。将传代培养的PGC用类黄酮单独攻击，或与CEF上产生活性氧物质（ROS）的系统联合攻击48小时。结果显示，CEF的支持效果优于GCF。类黄酮（1microg / ml）显着促进PGC增殖，这可能被ROS明显抑制。 ROS的氧化损伤进一步由超氧化物歧化酶活性和谷胱甘肽水平降低所证实。此外，毛喉素激活蛋白激酶A（PKA）可以显着刺激PGC的增殖，但是PKA抑制剂H89可以抑制DAI和QUE诱导的增殖作用。这些结果表明，培养的PGC对外源剂对增殖有反应，抗氧化剂类黄酮可通过其涉及PKA信号途径的抗氧化剂作用而恢复细胞内抗氧化剂系统并促进PGC增殖。

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