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Differentiation of AFS cells derived from the EGFP gene transgenic porcine fetuses.

机译:衍生自EGFP基因转基因猪胎儿的AFS细胞的分化。

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摘要

We have obtained the EGFP (enhanced green fluorescence protein) gene transgenic porcine fetuses before. The aims of this study were (i) to determine whether stem cells could be isolated from amniotic fluid of the transgenic porcine fetuses, and (ii) to determine if these stem cells could express EGFP and differentiate in vitro. The results demonstrated that stem cells could be isolated from amniotic fluid of the EGFP gene transgenic porcine fetuses and could express EGFP and differentiate in vitro. Undifferentiated AFSs (amniotic fluid-derived stem cells) expressed POU5F1, THY1 and SOX2, while the following differentiation cells expressed markers for chondrogenic (COL2A1), osteogenic (osteocalcin and osteonectin) and neurogenic cells such as astrocyte (GFAP), oligodendrocyte (GALC) and neuron (NF, ENO2 and MAP).
机译:我们之前已经获得了EGFP(增强型绿色荧光蛋白)基因转基因猪胎儿。这项研究的目的是(i)确定是否可以从转基因猪胎儿的羊水中分离出干细胞,以及(ii)确定这些干细胞是否可以表达EGFP并在体外分化。结果表明,可以从EGFP基因转基因猪胎儿的羊水中分离出干细胞,并且可以表达EGFP并在体外分化。未分化的AFS(羊水来源的干细胞)表达POU5F1,THY1和SOX2,而随后的分化细胞表达软骨生成(COL2A1),成骨(骨钙蛋白和骨连接蛋白)和神经源细胞(如星形胶质细胞(GFAP),少突胶质细胞(GALC))的标记和神经元(NF,ENO2和MAP)。

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