• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Gelatin induces trophectoderm differentiation of mouse embryonic stem cells.
【24h】

Gelatin induces trophectoderm differentiation of mouse embryonic stem cells.

机译：明胶诱导小鼠胚胎干细胞的滋养外胚层分化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we selected gelatin as ECM (extracellular matrix) to support differentiation of mES (mouse embryonic stem) cells into TE (trophectoderm), as gelatin was less expensive and widely used. We found that 0.2% and 1.5% gelatin were the suitable concentrations to induce TE differentiation by means of detecting Cdx2 expression using real-time PCR. Moreover, about 15% cells were positive for Cdx2 staining after 6 days differentiation. We discovered that the expressions of specific markers for TE, such as Cdx2, Eomes, Hand1 and Esx1 were prominently increased after gelatin induction. Meanwhile, the expression of Oct4 was significantly decreased. We also found that inhibition of the BMP (bone morphogenetic protein) signalling by Noggin could promote mES cells differentiation into TE, whereas inhibition of the Wnt signalling by Dkk1 had the contrary effect. This could be used as a tool to study the differentiation and function of early trophoblasts as well as further elucidating the molecular mechanism during abnormal placental development.
机译：在这项研究中，我们选择明胶作为ECM（细胞外基质）来支持mES（小鼠胚胎干）细胞分化为TE（滋养外胚层），因为明胶价格便宜且用途广泛。我们发现0.2％和1.5％的明胶是通过使用实时PCR检测Cdx2表达来诱导TE分化的合适浓度。此外，分化6天后约15％的细胞对Cdx2染色呈阳性。我们发现明胶诱导后，TE的特定标志物（如Cdx2，Eomes，Hand1和Esx1）的表达显着增加。同时，Oct4的表达明显降低。我们还发现，Noggin抑制BMP（骨形态发生蛋白）信号传导可促进mES细胞分化为TE，而Dkk1抑制Wnt信号传导则具有相反的作用。这可以用作研究早期滋养细胞的分化和功能以及进一步阐明异常胎盘发育过程中分子机制的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号