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首页> 外文期刊>Cell and Tissue Research >Defining the morphological phenotype: 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is a novel marker for in situ detection of canine but not rat olfactory ensheathing cells.
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Defining the morphological phenotype: 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is a novel marker for in situ detection of canine but not rat olfactory ensheathing cells.

机译：定义形态表型：2'，3'-环核苷酸3'-磷酸二酯酶（CNPase）是用于原位检测犬而不是大鼠嗅鞘细胞的新型标记物。

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Olfactory ensheathing cells (OECs) are the non-myelinating glial cells of the olfactory nerves and bulb. The fragmentary characterization of OECs in situ during normal development may be due to their small size requiring intricate ultrastructural analysis and to the fact that available markers for in situ detection are either expressed only by OEC subpopulations or lost during development. In the present study, we searched for markers with stable expression in OECs and investigated the spatiotemporal distribution of CNPase, an early oligodendrocyte/Schwann cell marker, in comparison with the prototype marker p75(NTR). Anti-CNPase antibodies labeled canine but not rat OECs in situ, while Schwann cells and oligodendrocytes were positive in both species. CNPase immunoreactivity in the dog was confined to all OECs throughout the postnatal development and associated with the entire cell body, including its finest processes, while p75(NTR) was mainly detected in perineural cells and only in some neonatal OECs. Adult olfactory bulb slices displayed CNPase expression after 4 and 10 days, while p75(NTR) was detectable only after 10 days in vitro. Finally, treatment of purified adult canine OECs with fibroblast growth factor-2 significantly reduced CNPase expression at the protein and mRNA level. Taken together, we conclude that CNPase but not p75(NTR) is a stable marker suitable for in situ visualization of OECs that will facilitate their light-microscopic characterization and challenge our general view of OEC marker expression in situ. The fact that canine but not rat OECs expressed CNPase supports the idea that glia from large animals differs substantially from rodents.
机译：嗅鞘细胞（OEC）是嗅神经和鳞茎的非髓鞘神经胶质细胞。正常发育过程中OEC的原位表征可能是由于其体积小，需要进行复杂的超微结构分析，以及用于原位检测的可用标记仅由OEC亚群表达或在发育过程中丢失的事实。在本研究中，我们搜索了在OEC中稳定表达的标志物，并与原型标志物p75（NTR）比较，研究了早期少突胶质细胞/ Schwann细胞标志物CNPase的时空分布。抗CNPase抗体标记犬而不是原位大鼠OEC，而雪旺氏细胞和少突胶质细胞在这两个物种中均为阳性。狗中的CNPase免疫反应性仅限于整个产后发育中的所有OEC，并且与整个细胞体有关，包括其最精细的过程，而p75（NTR）主要在神经周细胞中检出，仅在某些新生儿OEC中检出。成人嗅球切片在第4天和第10天后显示CNPase表达，而仅在体外第10天后才检测到p75（NTR）。最后，用成纤维细胞生长因子2处理纯化的成年犬OEC可以在蛋白质和mRNA水平上显着降低CNPase表达。两者合计，我们得出结论，CNPase，而不是p75（NTR）是一种稳定的标记物，适用于OEC的原位可视化，这将有助于它们的光学显微镜表征，并挑战我们对OEC标记原位表达的一般看法。犬而不是大鼠OEC表达CNPase的事实支持了这样的观点，即大型动物的胶质细胞与啮齿动物完全不同。

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