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首页> 外文期刊>Cell and Tissue Research >IL-17 and FGF signaling involved in mouse mesenchymal stem cell proliferation.
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IL-17 and FGF signaling involved in mouse mesenchymal stem cell proliferation.

机译：IL-17和FGF信号传导参与小鼠间充质干细胞增殖。

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The mouse is a suitable experimental model to study the biology of mesenchymal stem cells (MSCs), as well as to be used in biocompatibility studies and tissue engineering models. However, the isolation and purification of murine MSCs is far more challenging than their counterparts from other species. In this study, we isolated, expanded and characterized mouse MSCs from bone marrow (BM-MSCs). Additionally, we analyzed the effects of two regulatory molecules, interleukin 17 (IL-17) and basic fibroblast growth factor (bFGF), on BM-MSCs growth and elucidated the signaling pathways involved. The results revealed that IL-17 increased the frequency of colony-forming units fibroblast (CFU-F) as well as the BM-MSCs proliferation in a dose-dependent manner, while bFGF supplementation had no significant effect on CFU-F frequency but induced an increase in cell proliferation. Their combined usage did not produce additive effects on BM-MSCs proliferation and even induced reduction in the number of CFU-F. Also, the involvement of both p38 and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinases (MAPKs) signaling in proliferative activity of IL-17 and bFGF on murine BM-MSCs and, moreover, the increased co-activation of a common signaling molecule, p38 MAPK, were demonstrated. Together, the data presented highlighted the role of IL-17 and bFGF in murine BM-MSCs proliferation and pointed to the complexity and specificity of the signaling networks leading to MSCs proliferation in response to different regulatory molecules.
机译：小鼠是研究间充质干细胞（MSCs）生物学的合适实验模型，也可用于生物相容性研究和组织工程模型。但是，鼠MSC的分离和纯化要比其他物种的MSC更具挑战性。在这项研究中，我们从骨髓（BM-MSC）中分离，扩增和表征了小鼠MSC。此外，我们分析了两种调节分子白介素17（IL-17）和碱性成纤维细胞生长因子（bFGF）对BM-MSCs生长的影响，并阐明了所涉及的信号通路。结果表明，IL-17以剂量依赖的方式增加了集落形成单位成纤维细胞（CFU-F）的频率以及BM-MSC的增殖，而补充bFGF对CFU-F的频率没有显着影响，但诱导细胞增殖的增加。它们的组合使用不会对BM-MSC的增殖产生累加作用，甚至不会引起CFU-F数量的减少。此外，p38和细胞外信号调节激酶（ERK）丝裂原激活的蛋白激酶（MAPKs）信号都参与了IL-17和bFGF对鼠BM-MSC的增殖活性，此外，α38的共激活增加证明了常见的信号分子，p38 MAPK。在一起提供的数据突出了IL-17和bFGF在鼠BM-MSC增殖中的作用，并指出了导致MSC响应不同调控分子增殖的信号网络的复杂性和特异性。

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