• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Human neutrophil-derived elastase induces airway smooth muscle cell proliferation
【24h】

Human neutrophil-derived elastase induces airway smooth muscle cell proliferation

机译：人类中性粒细胞衍生的弹性蛋白酶诱导气道平滑肌细胞增殖

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Neutrophils and their derived elastase are abundant in chronic inflammatory responses of asthma. This study aimed to investigate the mitogenic effect of elastase on airway smooth muscle (ASM) cells and the implicated signal transduction pathway. Near confluent cultured human ASM cells were treated with human neutrophil elastase (HNE, 0.01 to 0.5 mug/ml) or vehicle for 24 hours with or without extracellular signal-regulated kinase (ERK) inhibitor (PD98059, 30 muM), p38 kinase inhibitor (SB203580, 10 muM) or elastase inhibitor II (100 mug/ml). The ASM cell numbers were counted by a hemocytometer and DNA synthesis was assessed by flowcytometry. Western blots analysis for the expression of ERK, p38 and cyclin D1 was determined. HNE dose-dependently increased ASM cell numbers and the percentage of cells entering S-phase of cell cycle. This response was abolished by neutrophil elastase inhibitors and attenuated by PD98059, but not SB203580. HNE increased ERK phosphorylation and cyclin D-1 expression. Pretreatment with PD98059 significantly inhibited elastase-induced cyclin D1 activity. The increased ASM cellular gap and cell shape change by proteolytic activity of HNE may be contributory to ERK activation and therefore cell proliferation. Our results demonstrate that HNE is mitogenic for ASM cells by increasing cyclin D1 activity through ERK signaling pathway. (C) 2004 Elsevier Inc. All rights reserved. [References: 57]
机译：中性粒细胞及其衍生的弹性蛋白酶在哮喘的慢性炎症反应中含量很高。这项研究旨在调查弹性蛋白酶对气道平滑肌（ASM）细胞的促有丝分裂作用及其信号转导途径。将近汇合培养的人ASM细胞用人嗜中性粒细胞弹性蛋白酶（HNE，0.01至0.5 mug / ml）或赋形剂处理24小时，有或没有细胞外信号调节激酶（ERK）抑制剂（PD98059，30μM），p38激酶抑制剂（ SB203580，10μM）或弹性蛋白酶抑制剂II（100杯/毫升）。通过血细胞计数器计数ASM细胞数，并通过流式细胞术评估DNA合成。确定了ERK，p38和cyclin D1表达的蛋白质印迹分析。 HNE剂量依赖性地增加了ASM细胞数量和进入细胞周期S期的细胞百分比。中性粒细胞弹性蛋白酶抑制剂消除了该反应，PD98059减弱了该反应，但SB203580没有。 HNE增加ERK磷酸化和细胞周期蛋白D-1表达。用PD98059预处理可显着抑制弹性蛋白酶诱导的细胞周期蛋白D1活性。通过HNE的蛋白水解活性增加的ASM细胞间隙和细胞形状变化可能是ERK活化并因此促进细胞增殖的原因。我们的结果表明，HNE通过ERK信号通路增加细胞周期蛋白D1的活性，从而对ASM细胞有丝分裂作用。 （C）2004 Elsevier Inc.保留所有权利。 [参考：57]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号