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Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549

机译：半枝莲提取物对人肺癌细胞A549的抗癌活性及其机制

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摘要

Scutellaria barbata (S. barbata), a traditional Chinese herbal medicine native to southern China, is widely used as an anti-inflammatory and a diuretic in China. Several studies have indicated that extracts of S. barbata have growth inhibitory effects on a number of human cancers. Treatment of lung cancer, digestive system cancers, hepatoma, breast cancer, and chorioepithelioma by S. barbata extracts was reported. However, the mechanism underlying the antitumor activity was unclear. In this study, we studied the growth inhibitory effect of S. barbata and determined its mechanism of antitumor activity using human lung cancer cell line A549. Our results showed that ethanol extracts of S. barbata greatly inhibited A549 cell growth, with IC50 of 0.21 mg/ml. The major mechanisms of inhibition included cell apoptosis and cytotoxic effects. cDNA microarray analysis showed that 16 genes, involved in DNA damage, cell cycle control, nucleic acid binding and protein phosphorylation, underwent more than 5-fold change. These data indicated that these processes are involved in S. barbata-mediated killing of cancer cells. A surprising finding is that CD209, related to dendritic cell (DC) function, was dramatically downregulated by 102-fold. Further functional studies are needed to assess the role of the array-identified genes in S. barbata mediated anticancer activity. (C) 2004 Elsevier Inc. All rights reserved.
机译：黄cut（S。barbata）是中国南方的中草药，在中国被广泛用作消炎药和利尿剂。数项研究表明，半枝莲提取物对许多人类癌症具有生长抑制作用。据报道，巴巴球菌提取物可治疗肺癌，消化系统癌，肝癌，乳腺癌和绒毛膜上皮瘤。但是，抗肿瘤活性的机制尚不清楚。在这项研究中，我们研究了巴氏酵母的生长抑制作用，并确定了使用人肺癌细胞系A549的抗肿瘤活性的机制。我们的结果表明，半枝莲的乙醇提取物极大地抑制了A549细胞的生长，IC50为0.21 mg / ml。抑制的主要机制包括细胞凋亡和细胞毒性作用。 cDNA微阵列分析表明，涉及DNA损伤，细胞周期控制，核酸结合和蛋白质磷酸化的16个基因发生了5倍以上的变化。这些数据表明，这些过程参与了半枝莲（S.barbata）介导的癌细胞杀伤。令人惊讶的发现是与树突状细胞（DC）功能相关的CD209被大幅下调了102倍。需要进一步的功能研究来评估阵列鉴定的基因在半枝莲中介导的抗癌活性中的作用。 （C）2004 Elsevier Inc.保留所有权利。

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