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PARACRINE FUNCTION OF ENDOTHELIUM-DERIVED NITRIC OXIDE

机译：内皮素衍生的一氧化氮的PARRACINE功能

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We explored whether endothelium-derived nitric oxide (EDNO) acts in an autocrine or paracrine fashion to activate the soluble guanylate cyclase by measuring the elevations of cGMP levels. Comparisons were made between two culture models in which EDNO can stimulate cGMP synthesis within the same cells (endothelial cells) where it is formed, or in the neighboring cells (smooth muscle cells). The basal amount of EDNO showed no difference in cGMP levels when the endothelial cells (EC) were cultured either alone (iso-culture) or together with the smooth muscle cells (SMC, co-culture). However, cGMP levels were synergistically increased by the BK-stimulated EDNO when EC were co-cultured with SMC. The synergistical increase was significantly inhibited by 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186), an extracellular radical scavenger. These findings suggest that the tonic formed basal EDNO has an autocrine effect on cGMP levels of EC themselves. In contrast, BK-stimulated phasic formed EDNO has not only an autocrine effect, but also a paracrine effect on cGMP levels of EC themselves and the adjacent SMC. [References: 27]
机译：我们探讨了内皮源性一氧化氮（EDNO）是否以自分泌或旁分泌的方式通过测量cGMP水平的升高来激活可溶性鸟苷酸环化酶。在两种培养模型之间进行了比较，其中EDNO可以刺激形成cGMP的同一细胞（内皮细胞）或邻近细胞（平滑肌细胞）内的cGMP合成。当内皮细胞（EC）单独（同种培养）或与平滑肌细胞（SMC，共培养）一起培养时，EDNO的基础量在cGMP水平上没有差异。然而，当EC与SMC共培养时，BK刺激的EDNO协同增加cGMP水平。协同增加被细胞外自由基清除剂3-甲基-1-苯基-2-吡唑啉-5-酮（MCI-186）显着抑制。这些发现表明补品形成的基础EDNO对EC本身的cGMP水平具有自分泌作用。相反，BK刺激的相形成的EDNO不仅具有自分泌作用，而且还对EC自身和相邻SMC的cGMP水平具有旁分泌作用。 [参考：27]

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